| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.114ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This DGAT1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
DGAT1 |
| Gene ID: |
8694 |
| Uniprot ID: |
DGAT1_HUMAN |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats. In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage. Also present in female mammary glands, where it produces fat in the milk. May be involved in VLDL (very low density lipoprotein) assembly. In contrast to DGAT2 it is not essential for survival. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders. Exhibits additional acyltransferase activities, includin acyl CoA:monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester synthases. Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG). |
| Protein Name | Diacylglycerol O-Acyltransferase 1Acat-Related Gene Product 1Acyl-Coa Retinol O-Fatty-AcyltransferaseAratRetinol O-Fatty-AcyltransferaseDiglyceride Acyltransferase |
| Database Links | Reactome: R-HSA-1482883Reactome: R-HSA-6798695Reactome: R-HSA-75109 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative ELISA Names | Diacylglycerol O-Acyltransferase 1 ELISA kitAcat-Related Gene Product 1 ELISA kitAcyl-Coa Retinol O-Fatty-Acyltransferase ELISA kitArat ELISA kitRetinol O-Fatty-Acyltransferase ELISA kitDiglyceride Acyltransferase ELISA kitDGAT1 ELISA kitAGRP1 ELISA kitDGAT ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
