• Human DEFA1; DEFA1B (Neutrophil defensin 1) Sandwich ELISA (STJE0002513)

Human DEFA1; DEFA1B (Neutrophil defensin 1) Sandwich ELISA (STJE0002513)

SKU:
STJE0002513

Shipping:
Free Shipping
Current Stock:
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This DEFA1; DEFA1B Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: DEFA1.DEFA1B
Gene ID: 1667/728358
Uniprot ID: DEF1_HUMAN
Specificity: This kit recognizes Human DEF Alpha 1 in samples. No significant cross-reactivity or interference between Human DEF Alpha 1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Effector molecule of the innate immune system that acts via antibiotic-like properties against a broad array of infectious agents including bacteria, fungi, and viruses or by promoting the activation and maturation of some APCs. Interacts with the essential precursor of cell wall synthesis lipid II to inhibit bacterial cell wall synthesis. Inhibits adenovirus infection via inhibition of viral disassembly at the vertex region, thereby restricting the release of internal capsid protein pVI, which is required for endosomal membrane penetration during cell entry. In addition, interaction with adenovirus capsid leads to the redirection of viral particles to TLR4 thereby promoting a NLRP3-mediated inflammasome response and interleukin 1-beta (IL-1beta) release. Induces the production of proinflammatory cytokines including type I interferon (IFN) in plasmacytoid dendritic cells (pDCs) by triggering the degradation of NFKBIA and nuclear translocation of IRF1, both of which are required for activation of pDCs.
Protein Name Neutrophil Defensin 1
Defensin - Alpha 1
Hnp-1
Hp-1
Hp1 Cleaved Into - Hp 1-56 - Neutrophil Defensin 2
Hnp-2
Hp-2
Hp2
Database Links Reactome: R-HSA-1461973
Reactome: R-HSA-1462054
Reactome: R-HSA-6798695
Cellular Localisation Secreted
Alternative ELISA Names Neutrophil Defensin 1 ELISA kit
Defensin - Alpha 1 ELISA kit
Hnp-1 ELISA kit
Hp-1 ELISA kit
Hp1 Cleaved Into - Hp 1-56 - Neutrophil Defensin 2 ELISA kit
Hnp-2 ELISA kit
Hp-2 ELISA kit
Hp2 ELISA kit
DEFA1 ELISA kit
DEF1 ELISA kit
DEFA2 ELISA kit
MRS.DEFA1B ELISA kit
Specificity This kit recognizes Human DEFα1 in samples. No significant cross-reactivity or interference between Human DEFα1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-97 91
EDTA plasma (n=8) 93-107 99
Cell culture media (n=8) 94-111 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 98.61 179.14 867.81 97.17 195.46 814.62
Standard deviation 6.80 8.12 40.79 6.08 11.00 43.66
CV (%) 6.90 4.53 4.70 6.26 5.63 5.36
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance