| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.062ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This DDX5 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
DDX5 |
| Gene ID: |
1655 |
| Uniprot ID: |
DDX5_HUMAN |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Arg-502 is dimethylated, probably to asymmetric dimethylarginine. Sumoylated.sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination. Polyubiquitinated, leading to proteasomal degradation. Weakly phosphorylated in the G1/S phase of the cell cycle and much more at G2/M, especially at Thr and Tyr residues. |
| Function | Involved in the alternative regulation of pre-mRNA splicing.its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation.the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner.the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms. |
| Protein Name | Probable Atp-Dependent Rna Helicase Ddx5Dead Box Protein 5Rna Helicase P68 |
| Database Links | Reactome: R-HSA-3899300Reactome: R-HSA-72163Reactome: R-HSA-9018519Reactome: R-HSA-9682706Reactome: R-HSA-9694686 |
| Cellular Localisation | NucleusNucleolusNucleus SpeckleCytoplasmDuring The G0 PhasePredominantly Located In The NucleusCytoplasmic Levels Increase During The G1/S PhaseDuring The M PhaseLocated At The Vicinity Of The Condensed ChromosomesAt G1Localizes In The Cytoplasm |
| Alternative ELISA Names | Probable Atp-Dependent Rna Helicase Ddx5 ELISA kitDead Box Protein 5 ELISA kitRna Helicase P68 ELISA kitDDX5 ELISA kitG17P1 ELISA kitHELR ELISA kitHLR1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
