| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.138ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This DDIT3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
DDIT3 |
| Gene ID: |
1649 |
| Uniprot ID: |
DDIT3_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Ubiquitinated, leading to its degradation by the proteasome. Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity. |
| Function | Multifunctional transcription factor in endoplasmic reticulum (ER) stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates.expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Together with ATF4, mediates ER-mediated cell death by promoting expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the unfolded protein response (UPR) in response to ER stress. Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response. Acts as a major regulator of postnatal neovascularization through regulation of endothelial nitric oxide synthase (NOS3)-related signaling. |
| Protein Name | Dna Damage-Inducible Transcript 3 ProteinDdit-3C/Ebp ZetaC/Ebp-Homologous ProteinChopC/Ebp-Homologous Protein 10Chop-10Ccaat/Enhancer-Binding Protein Homologous ProteinGrowth Arrest And Dna Damage-Inducible Protein Gadd153 |
| Database Links | Reactome: R-HSA-380994Reactome: R-HSA-381183Reactome: R-HSA-9614657Reactome: R-HSA-9633012Reactome: R-HSA-9648895 |
| Cellular Localisation | CytoplasmNucleusPresent In The Cytoplasm Under Non-Stressed Conditions And Er Stress Leads To Its Nuclear Accumulation |
| Alternative ELISA Names | Dna Damage-Inducible Transcript 3 Protein ELISA kitDdit-3 ELISA kitC/Ebp Zeta ELISA kitC/Ebp-Homologous Protein ELISA kitChop ELISA kitC/Ebp-Homologous Protein 10 ELISA kitChop-10 ELISA kitCcaat/Enhancer-Binding Protein Homologous Protein ELISA kitGrowth Arrest And Dna Damage-Inducible Protein Gadd153 ELISA kitDDIT3 ELISA kitCHOP ELISA kitCHOP10 ELISA kitGADD153 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
