| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88ng/mL |
| Detection Limit: |
3.13~200ng/mL |
| Short Description: |
This DAO Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
AOC1 |
| Gene ID: |
26 |
| Uniprot ID: |
AOC1_HUMAN |
| Specificity: |
This kit recognizes Human DAO in samples. No significant cross-reactivity or interference between Human DAO and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue. |
| Function | Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function. |
| Protein Name | Amiloride-Sensitive Amine Oxidase Copper-ContainingDaoDiamine OxidaseAmiloride-Binding Protein 1Amine Oxidase Copper Domain-Containing Protein 1HistaminaseKidney Amine OxidaseKao |
| Database Links | Reactome: R-HSA-211945Reactome: R-HSA-6798695 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative ELISA Names | Amiloride-Sensitive Amine Oxidase Copper-Containing ELISA kitDao ELISA kitDiamine Oxidase ELISA kitAmiloride-Binding Protein 1 ELISA kitAmine Oxidase Copper Domain-Containing Protein 1 ELISA kitHistaminase ELISA kitKidney Amine Oxidase ELISA kitKao ELISA kitAOC1 ELISA kitABP1 ELISA kitDAO1 ELISA kit |
| Specificity | This kit recognizes Human DAO in samples. No significant cross-reactivity or interference between Human DAO and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-106 | 98 |
| EDTA plasma (n=8) | 85-100 | 92 |
| Cell culture media (n=8) | 89-102 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 10.74 | 28.26 | 90.38 | 10.95 | 27.59 | 89.37 |
| Standard deviation | 0.68 | 1.29 | 3.07 | 0.64 | 1.43 | 3.93 |
| CV (%) | 6.33 | 4.56 | 3.40 | 5.84 | 5.18 | 4.40 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
