• Human CYP7A1 (Cytochrome P450 7A1) Sandwich ELISA Kit Ready-To-Use (STJE0014702)

Human CYP7A1 (Cytochrome P450 7A1) Sandwich ELISA Kit Ready-To-Use (STJE0014702)

SKU:
STJE0014702

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.34ng/mL
Detection Limit: 0.781-50ng/mL
Short Description: This CYP7A1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: CYP7A1
Gene ID: 1581
Uniprot ID: CP7A1_HUMAN
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity Detected in liver.
Post Translational Modifications
Function A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis. 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives.
Protein Name Cytochrome P450 7a1
24-Hydroxycholesterol 7-Alpha-Hydroxylase
Cypvii
Cholesterol 7-Alpha-Hydroxylase
Cholesterol 7-Alpha-Monooxygenase
Database Links Reactome: R-HSA-192105
Reactome: R-HSA-193368
Reactome: R-HSA-193807
Reactome: R-HSA-1989781
Reactome: R-HSA-211976
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Microsome Membrane
Alternative ELISA Names Cytochrome P450 7a1 ELISA kit
24-Hydroxycholesterol 7-Alpha-Hydroxylase ELISA kit
Cypvii ELISA kit
Cholesterol 7-Alpha-Hydroxylase ELISA kit
Cholesterol 7-Alpha-Monooxygenase ELISA kit
CYP7A1 ELISA kit
CYP7 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance