| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.128ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This CYP3A4 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
CYP3A4 |
| Gene ID: |
1576 |
| Uniprot ID: |
CP3A4_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Expressed in prostate and liver. According to some authors, it is not expressed in brain. According to others, weak levels of expression are measured in some brain locations. Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level). |
| Post Translational Modifications | Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro). |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C-16 position. Plays a role in the metabolism of androgens, particularly in oxidative deactivation of testosterone. Metabolizes testosterone to less biologically active 2beta- and 6beta-hydroxytestosterones. Contributes to the formation of hydroxycholesterols (oxysterols), particularly A-ring hydroxylated cholesterol at the C-4beta position, and side chain hydroxylated cholesterol at the C-25 position, likely contributing to cholesterol degradation and bile acid biosynthesis. Catalyzes bisallylic hydroxylation of polyunsaturated fatty acids (PUFA). Catalyzes the epoxidation of double bonds of PUFA with a preference for the last double bond. Metabolizes endocannabinoid arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling. Plays a role in the metabolism of retinoids. Displays high catalytic activity for oxidation of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA). Further metabolizes atRA toward 4-hydroxyretinoate and may play a role in hepatic atRA clearance. Responsible for oxidative metabolism of xenobiotics. Acts as a 2-exo-monooxygenase for plant lipid 1,8-cineole (eucalyptol). Metabolizes the majority of the administered drugs. Catalyzes sulfoxidation of the anthelmintics albendazole and fenbendazole. Hydroxylates antimalarial drug quinine. Acts as a 1,4-cineole 2-exo-monooxygenase. Also involved in vitamin D catabolism and calcium homeostasis. Catalyzes the inactivation of the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)). |
| Protein Name | Cytochrome P450 3a41 -4-Cineole 2-Exo-Monooxygenase1 -8-Cineole 2-Exo-MonooxygenaseAlbendazole MonooxygenaseSulfoxide-FormingAlbendazole SulfoxidaseCypiiia3Cypiiia4Cholesterol 25-HydroxylaseCytochrome P450 3a3Cytochrome P450 HlpCytochrome P450 Nf-25Cytochrome P450-Pcn1Nifedipine OxidaseQuinine 3-Monooxygenase |
| Database Links | Reactome: R-HSA-211945Reactome: R-HSA-211981Reactome: R-HSA-5423646Reactome: R-HSA-9027307Reactome: R-HSA-9749641Reactome: R-HSA-9754706Reactome: R-HSA-9757110 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMicrosome Membrane |
| Alternative ELISA Names | Cytochrome P450 3a4 ELISA kit1 -4-Cineole 2-Exo-Monooxygenase ELISA kit1 -8-Cineole 2-Exo-Monooxygenase ELISA kitAlbendazole Monooxygenase ELISA kitSulfoxide-Forming ELISA kitAlbendazole Sulfoxidase ELISA kitCypiiia3 ELISA kitCypiiia4 ELISA kitCholesterol 25-Hydroxylase ELISA kitCytochrome P450 3a3 ELISA kitCytochrome P450 Hlp ELISA kitCytochrome P450 Nf-25 ELISA kitCytochrome P450-Pcn1 ELISA kitNifedipine Oxidase ELISA kitQuinine 3-Monooxygenase ELISA kitCYP3A4 ELISA kitCYP3A3 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
