| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.234ng/mL |
| Detection Limit: |
0.625-40ng/mL |
| Short Description: |
This CYP2C9 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
CYP2C9 |
| Gene ID: |
1559 |
| Uniprot ID: |
CP2C9_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and steroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. Exhibits low catalytic activity for the formation of catechol estrogens from 17beta-estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2. Catalyzes bisallylic hydroxylation and hydroxylation with double-bond migration of polyunsaturated fatty acids (PUFA). Also metabolizes plant monoterpenes such as limonene. Oxygenates (R)- and (S)-limonene to produce carveol and perillyl alcohol. Contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan. |
| Protein Name | Cytochrome P450 2c9(R-Limonene 6-Monooxygenase(S-Limonene 6-Monooxygenase(S-Limonene 7-MonooxygenaseCypiic9Cholesterol 25-HydroxylaseCytochrome P-450mpCytochrome P450 Mp-4Cytochrome P450 Mp-8Cytochrome P450 Pb-1S-Mephenytoin 4-Hydroxylase |
| Database Links | Reactome: R-HSA-211981Reactome: R-HSA-211999Reactome: R-HSA-2142670Reactome: R-HSA-2142816Reactome: R-HSA-9027307Reactome: R-HSA-9749641 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinMicrosome Membrane |
| Alternative ELISA Names | Cytochrome P450 2c9 ELISA kit(R-Limonene 6-Monooxygenase ELISA kit(S-Limonene 6-Monooxygenase ELISA kit(S-Limonene 7-Monooxygenase ELISA kitCypiic9 ELISA kitCholesterol 25-Hydroxylase ELISA kitCytochrome P-450mp ELISA kitCytochrome P450 Mp-4 ELISA kitCytochrome P450 Mp-8 ELISA kitCytochrome P450 Pb-1 ELISA kitS-Mephenytoin 4-Hydroxylase ELISA kitCYP2C9 ELISA kitCYP2C10 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
