| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CYP26A1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CYP26A1 |
| Gene ID: |
1592 |
| Uniprot ID: |
CP26A_HUMAN |
| Specificity: |
This kit recognizes Human CYP26A1 in samples. No significant cross-reactivity or interference between Human CYP26A1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in most fetal and adult tissues with highest levels in adult liver, heart, pituitary gland, adrenal gland, placenta and regions of the brain. Expressed at high levels in lung, pancreas, skin and uterus (at protein level). Lower expression level is detected in spleen, kidney, intestine and adipose tissue (at protein level). |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of retinoates (RAs), the active metabolites of vitamin A, and critical signaling molecules in animals. RAs exist as at least four different isomers: all-trans-RA (atRA), 9-cis-RA, 13-cis-RA, and 9,13-dicis-RA, where atRA is considered to be the biologically active isomer, although 9-cis-RA and 13-cis-RA also have activity (Probable). Catalyzes the hydroxylation of atRA primarily at C-4 and C-18, thereby contributing to the regulation of atRA homeostasis and signaling. Hydroxylation of atRA limits its biological activity and initiates a degradative process leading to its eventual elimination (Probable). Involved in the convertion of atRA to all-trans-4-oxo-RA. Able to metabolize other RAs such as 9-cis, 13-cis and 9,13-di-cis RA. Can oxidize all-trans-13,14-dihydroretinoate (DRA) to metabolites which could include all-trans-4-oxo-DRA, all-trans-4-hydroxy-DRA, all-trans-5,8-epoxy-DRA, and all-trans-18-hydroxy-DRA. May play a role in the oxidative metabolism of xenobiotics such as tazarotenic acid. |
| Protein Name | Cytochrome P450 26a1Cyp26a1Cytochrome P450 Retinoic Acid-Inactivating 1Cytochrome P450raiHp450raiRetinoic Acid 4-HydroxylaseRetinoic Acid-Metabolizing Cytochrome |
| Database Links | Reactome: R-HSA-211916Reactome: R-HSA-5365859 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinMicrosome Membrane |
| Alternative CLIA Names | Cytochrome P450 26a1 CLIA kitCyp26a1 CLIA kitCytochrome P450 Retinoic Acid-Inactivating 1 CLIA kitCytochrome P450rai CLIA kitHp450rai CLIA kitRetinoic Acid 4-Hydroxylase CLIA kitRetinoic Acid-Metabolizing Cytochrome CLIA kitCYP26A1 CLIA kitCYP26 CLIA kitP450RAI1 CLIA kit |
| Specificity | This kit recognizes Human CYP26A1 in samples. No significant cross-reactivity or interference between Human CYP26A1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-106 | 98 |
| EDTA plasma(n=8) | 86-96 | 91 |
| Cell culture media(n=8) | 94-108 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 107.07 | 266.68 | 764.75 | 115.24 | 255.66 | 802.44 |
| Standard deviation | 11.86 | 24.53 | 68.14 | 12.50 | 27.87 | 70.29 |
| CV (%) | 11.08 | 9.20 | 8.91 | 10.85 | 10.90 | 8.76 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
