| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CYP1A2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CYP1A2 |
| Gene ID: |
1544 |
| Uniprot ID: |
CP1A2_HUMAN |
| Specificity: |
This kit recognizes Human CYP1A2 in samples. No significant cross-reactivity or interference between Human CYP1A2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Liver. |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer. Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Metabolizes caffeine via N3-demethylation (Probable). |
| Protein Name | Cytochrome P450 1a2Cypia2Cholesterol 25-HydroxylaseCytochrome P(3450Cytochrome P450 4Cytochrome P450-P3Hydroperoxy Icosatetraenoate Dehydratase |
| Database Links | Reactome: R-HSA-156581Reactome: R-HSA-211957Reactome: R-HSA-2142670Reactome: R-HSA-2142816Reactome: R-HSA-5423646Reactome: R-HSA-9018681Reactome: R-HSA-9027307 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinMicrosome Membrane |
| Alternative CLIA Names | Cytochrome P450 1a2 CLIA kitCypia2 CLIA kitCholesterol 25-Hydroxylase CLIA kitCytochrome P(3450 CLIA kitCytochrome P450 4 CLIA kitCytochrome P450-P3 CLIA kitHydroperoxy Icosatetraenoate Dehydratase CLIA kitCYP1A2 CLIA kit |
| Specificity | This kit recognizes Human CYP1A2 in samples. No significant cross-reactivity or interference between Human CYP1A2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-102 | 96 |
| EDTA plasma(n=8) | 84-95 | 90 |
| Cell culture media(n=8) | 88-103 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 101.25 | 297.42 | 900.22 | 95.56 | 319.21 | 920.75 |
| Standard deviation | 10.89 | 24.51 | 61.30 | 10.25 | 29.62 | 85.81 |
| CV (%) | 10.76 | 8.24 | 6.81 | 10.73 | 9.28 | 9.32 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
