| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.055ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This CYBb Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
CYBB |
| Gene ID: |
1536 |
| Uniprot ID: |
CY24B_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Detected in neutrophils (at protein level). |
| Post Translational Modifications | Glycosylated. Phosphorylated on Ser and Thr residues. Undergoes 'Lys-48'-linked polyubiquitination, likely by RNF145, triggering endoplasmic reticulum-associated degradation. |
| Function | Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. |
| Protein Name | Cytochrome B-245 Heavy ChainCgd91-PhoxCytochrome B(558 Subunit BetaCytochrome B558 Subunit BetaHeme-Binding Membrane Glycoprotein Gp91phoxNadph Oxidase 2Neutrophil Cytochrome B 91 Kda PolypeptideSuperoxide-Generating Nadph Oxidase Heavy Chain SubunitGp91-1Gp91-PhoxP22 Phagocyte B-Cytochrome |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-1236973Reactome: R-HSA-3299685Reactome: R-HSA-4420097Reactome: R-HSA-5668599Reactome: R-HSA-6798695Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinAs Unassembled Monomer May Localize To The Endoplasmic Reticulum |
| Alternative ELISA Names | Cytochrome B-245 Heavy Chain ELISA kitCgd91-Phox ELISA kitCytochrome B(558 Subunit Beta ELISA kitCytochrome B558 Subunit Beta ELISA kitHeme-Binding Membrane Glycoprotein Gp91phox ELISA kitNadph Oxidase 2 ELISA kitNeutrophil Cytochrome B 91 Kda Polypeptide ELISA kitSuperoxide-Generating Nadph Oxidase Heavy Chain Subunit ELISA kitGp91-1 ELISA kitGp91-Phox ELISA kitP22 Phagocyte B-Cytochrome ELISA kitCYBB ELISA kitNOX2 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
