| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This CXCL8 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CXCL8 |
| Gene ID: |
3576 |
| Uniprot ID: |
IL8_HUMAN |
| Specificity: |
This kit recognizes Human IL-8 in samples. No significant cross-reactivity or interference between Human IL-8 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways. |
| Protein Name | Interleukin-8Il-8C-X-C Motif Chemokine 8ChemokineC-X-C Motif Ligand 8EmoctakinGranulocyte Chemotactic Protein 1Gcp-1Monocyte-Derived Neutrophil Chemotactic FactorMdncfMonocyte-Derived Neutrophil-Activating PeptideMonapNeutrophil-Activating Protein 1Nap-1Protein 3-10cT-Cell Chemotactic Factor Cleaved Into - Mdncf-AGcp/Il-8 Protein IvIl8/Nap1 Form I - Interleukin-8(Ala-Il-877Gcp/Il-8 Protein IiIl-8(1-77Il8/Nap1 Form IiMdncf-B - Il-8(5-77 - Il-8(6-77(Ser-Il-872Gcp/Il-8 Protein IIl8/Nap1 Form IiiLymphocyte-Derived Neutrophil-Activating FactorLynapMdncf-CNeutrophil-Activating FactorNaf - Il-8(7-77Gcp/Il-8 Protein VIl8/Nap1 Form Iv - Il-8(8-77Gcp/Il-8 Protein ViIl8/Nap1 Form V - Il-8(9-77Gcp/Il-8 Protein IiiIl8/Nap1 Form Vi |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-375276Reactome: R-HSA-380108Reactome: R-HSA-380994Reactome: R-HSA-418594Reactome: R-HSA-6783783Reactome: R-HSA-6785807 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Interleukin-8 ELISA kitIl-8 ELISA kitC-X-C Motif Chemokine 8 ELISA kitChemokine ELISA kitC-X-C Motif Ligand 8 ELISA kitEmoctakin ELISA kitGranulocyte Chemotactic Protein 1 ELISA kitGcp-1 ELISA kitMonocyte-Derived Neutrophil Chemotactic Factor ELISA kitMdncf ELISA kitMonocyte-Derived Neutrophil-Activating Peptide ELISA kitMonap ELISA kitNeutrophil-Activating Protein 1 ELISA kitNap-1 ELISA kitProtein 3-10c ELISA kitT-Cell Chemotactic Factor Cleaved Into - Mdncf-A ELISA kitGcp/Il-8 Protein Iv ELISA kitIl8/Nap1 Form I - Interleukin-8 ELISA kit(Ala-Il-877 ELISA kitGcp/Il-8 Protein Ii ELISA kitIl-8(1-77 ELISA kitIl8/Nap1 Form Ii ELISA kitMdncf-B - Il-8(5-77 - Il-8(6-77 ELISA kit(Ser-Il-872 ELISA kitGcp/Il-8 Protein I ELISA kitIl8/Nap1 Form Iii ELISA kitLymphocyte-Derived Neutrophil-Activating Factor ELISA kitLynap ELISA kitMdncf-C ELISA kitNeutrophil-Activating Factor ELISA kitNaf - Il-8(7-77 ELISA kitGcp/Il-8 Protein V ELISA kitIl8/Nap1 Form Iv - Il-8(8-77 ELISA kitGcp/Il-8 Protein Vi ELISA kitIl8/Nap1 Form V - Il-8(9-77 ELISA kitGcp/Il-8 Protein Iii ELISA kitIl8/Nap1 Form Vi ELISA kitCXCL8 ELISA kitIL8 ELISA kit |
| Specificity | This kit recognizes Human IL-8 in samples. No significant cross-reactivity or interference between Human IL-8 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-99 | 94 |
| EDTA plasma (n=8) | 95-102 | 98 |
| Cell culture media (n=8) | 98-106 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 20.34 | 60.67 | 245.62 | 24.62 | 56.15 | 233.52 |
| Standard deviation | 1.21 | 2.80 | 12.26 | 1.39 | 2.80 | 9.69 |
| CV (%) | 5.95 | 4.62 | 4.99 | 5.65 | 4.99 | 4.15 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
