| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human CXCL6 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CXCL6 |
| Gene ID: |
6372 |
| Uniprot ID: |
CXCL6_HUMAN |
| Specificity: |
This kit recognizes Human GCP-2 in samples. No significant cross-reactivity or interference between Human GCP-2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7). |
| Protein Name | C-X-C Motif Chemokine 6Chemokine Alpha 3Cka-3Granulocyte Chemotactic Protein 2Gcp-2Small-Inducible Cytokine B6 Cleaved Into - Small-Inducible Cytokine B6 - N-Processed Variant 1 - Small-Inducible Cytokine B6 - N-Processed Variant 2 - Small-Inducible Cytokine B6 - N-Processed Variant 3 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | C-X-C Motif Chemokine 6 CLIA kitChemokine Alpha 3 CLIA kitCka-3 CLIA kitGranulocyte Chemotactic Protein 2 CLIA kitGcp-2 CLIA kitSmall-Inducible Cytokine B6 Cleaved Into - Small-Inducible Cytokine B6 - N-Processed Variant 1 - Small-Inducible Cytokine B6 - N-Processed Variant 2 - Small-Inducible Cytokine B6 - N-Processed Variant 3 CLIA kitCXCL6 CLIA kitGCP2 CLIA kitSCYB6 CLIA kit |
| Specificity | This kit recognizes Human GCP-2 in samples. No significant cross-reactivity or interference between Human GCP-2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 101-115 | 107 |
| EDTA plasma(n=8) | 88-103 | 96 |
| Cell culture media(n=8) | 98-110 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 52.25 | 109.38 | 412.58 | 54.03 | 117.58 | 449.60 |
| Standard deviation | 4.50 | 9.70 | 28.88 | 6.02 | 13.35 | 46.22 |
| CV (%) | 8.61 | 8.87 | 7.00 | 11.14 | 11.35 | 10.28 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
