| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This human CXCL2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CXCL2 |
| Gene ID: |
2920 |
| Uniprot ID: |
CXCL2_HUMAN |
| Specificity: |
This kit recognizes Human GRO Beta/CXCL2 in samples. No significant cross-reactivity or interference between Human GRO Beta/CXCL2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. |
| Function | Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. |
| Protein Name | C-X-C Motif Chemokine 2Growth-Regulated Protein BetaGro-BetaMacrophage Inflammatory Protein 2-AlphaMip2-Alpha Cleaved Into - Gro-Beta(5-73Gro-Beta-THematopoietic Synergistic FactorHsfSb-251353 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594Reactome: R-HSA-6783783 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | C-X-C Motif Chemokine 2 CLIA kitGrowth-Regulated Protein Beta CLIA kitGro-Beta CLIA kitMacrophage Inflammatory Protein 2-Alpha CLIA kitMip2-Alpha Cleaved Into - Gro-Beta(5-73 CLIA kitGro-Beta-T CLIA kitHematopoietic Synergistic Factor CLIA kitHsf CLIA kitSb-251353 CLIA kitCXCL2 CLIA kitGRO2 CLIA kitGROB CLIA kitMIP2A CLIA kitSCYB2 CLIA kit |
| Specificity | This kit recognizes Human GROβ/CXCL2 in samples. No significant cross-reactivity or interference between Human GROβ/CXCL2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-98 | 90 |
| EDTA plasma(n=8) | 87-104 | 95 |
| Cell culture media(n=8) | 95-111 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 24.06 | 51.86 | 188.79 | 26.45 | 54.81 | 174.47 |
| Standard deviation | 2.37 | 5.27 | 19.88 | 2.78 | 5.24 | 15.06 |
| CV (%) | 9.85 | 10.16 | 10.53 | 10.51 | 9.56 | 8.63 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
