| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human CXCL11 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CXCL11 |
| Gene ID: |
6373 |
| Uniprot ID: |
CXL11_HUMAN |
| Specificity: |
This kit recognizes Human I-TAC/CXCL11 in samples. No significant cross-reactivity or interference between Human I-TAC/CXCL11 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders. |
| Function | Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses. |
| Protein Name | C-X-C Motif Chemokine 11Beta-R1H174Interferon Gamma-Inducible Protein 9Ip-9Interferon-Inducible T-Cell Alpha ChemoattractantI-TacSmall-Inducible Cytokine B11 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | C-X-C Motif Chemokine 11 CLIA kitBeta-R1 CLIA kitH174 CLIA kitInterferon Gamma-Inducible Protein 9 CLIA kitIp-9 CLIA kitInterferon-Inducible T-Cell Alpha Chemoattractant CLIA kitI-Tac CLIA kitSmall-Inducible Cytokine B11 CLIA kitCXCL11 CLIA kitITAC CLIA kitSCYB11 CLIA kitSCYB9B CLIA kit |
| Specificity | This kit recognizes Human I-TAC/CXCL11 in samples. No significant cross-reactivity or interference between Human I-TAC/CXCL11 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 98-112 | 105 |
| EDTA plasma(n=8) | 87-99 | 94 |
| Cell culture media(n=8) | 96-111 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 51.41 | 115.32 | 466.51 | 55.92 | 108.70 | 461.95 |
| Standard deviation | 6.22 | 12.56 | 48.24 | 5.70 | 8.19 | 29.93 |
| CV (%) | 12.10 | 10.89 | 10.34 | 10.19 | 7.53 | 6.48 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
