| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5-4000pg/mL |
| Short Description: |
This CTSL Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CTSL |
| Gene ID: |
1514 |
| Uniprot ID: |
CATL1_HUMAN |
| Specificity: |
This kit recognizes Human CTSL in samples. No significant cross-reactivity or interference between Human CTSL and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen. In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter. In thymus, regulates CD4(+) T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells. Also mediates invariant chain processing in cortical thymic epithelial cells. Major elastin-degrading enzyme at neutral pH. Accumulates as a mature and active enzyme in the extracellular space of antigen presenting cells (APCs) to regulate degradation of the extracellular matrix in the course of inflammation. Secreted form generates endostatin from COL18A1. Critical for cardiac morphology and function. Plays an important role in hair follicle morphogenesis and cycling, as well as epidermal differentiation. Required for maximal stimulation of steroidogenesis by TIMP1. (Microbial infection) In cells lacking TMPRSS2 expression, facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via a slow acid-activated route with the proteolysis of coronavirus spike (S) glycoproteins in lysosome for entry into host cell. Proteolysis within lysosomes is sufficient to activate membrane fusion by coronaviruses SARS-CoV and EMC (HCoV-EMC) S as well as Zaire ebolavirus glycoproteins. Isoform 2: Functions in the regulation of cell cycle progression through proteolytic processing of the CUX1 transcription factor. Translation initiation at downstream start sites allows the synthesis of isoforms that are devoid of a signal peptide and localize to the nucleus where they cleave the CUX1 transcription factor and modify its DNA binding properties. |
| Protein Name | Procathepsin LCathepsin L1Major Excreted ProteinMep Cleaved Into - Cathepsin L - Cathepsin L Heavy Chain - Cathepsin L Light Chain |
| Database Links | Reactome: R-HSA-1236977Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1679131Reactome: R-HSA-2022090Reactome: R-HSA-2132295Reactome: R-HSA-8939242Reactome: R-HSA-9678110Reactome: R-HSA-9694614 |
| Cellular Localisation | LysosomeApical Cell MembranePeripheral Membrane ProteinExtracellular SideCytoplasmic VesicleSecretory VesicleChromaffin GranuleSecretedExtracellular SpaceLocalizes To The Apical Membrane Of Thyroid Epithelial CellsReleased At Extracellular Space By Activated Dendritic Cells And MacrophagesIsoform 2: NucleusTranslation Initiation At Downstream Start Sites Allows The Synthesis Of Isoforms That Are Devoid Of A Signal Peptide And Do Not Transit Through The Endoplasmic Reticulum To Localize To The NucleusNuclear Location Varies During The Cell CycleWith Higher Levels During S Phase |
| Alternative ELISA Names | Procathepsin L ELISA kitCathepsin L1 ELISA kitMajor Excreted Protein ELISA kitMep Cleaved Into - Cathepsin L - Cathepsin L Heavy Chain - Cathepsin L Light Chain ELISA kitCTSL ELISA kitCTSL1 ELISA kit |
| Specificity | This kit recognizes Human CTSL in samples. No significant cross-reactivity or interference between Human CTSL and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-102 | 93 |
| EDTA plasma (n=8) | 91-104 | 96 |
| Cell culture media (n=8) | 95-108 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 187.70 | 575.05 | 1881.18 | 171.62 | 561.21 | 1697.86 |
| Standard deviation | 11.11 | 28.69 | 87.47 | 11.50 | 30.25 | 66.56 |
| CV (%) | 5.92 | 4.99 | 4.65 | 6.70 | 5.39 | 3.92 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
