• Human CST3 (Cystatin-C) Sandwich ELISA (STJE0003797)
  • Human CST3 (Cystatin-C) Sandwich ELISA (STJE0003797)

Human CST3 (Cystatin-C) Sandwich ELISA (STJE0003797)

SKU:
STJE0003797

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This CST3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CST3
Gene ID: 1471
Uniprot ID: CYTC_HUMAN
Specificity: This kit recognizes Human Cys-C in samples. No significant cross-reactivity or interference between Human Cys-C and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
Function As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Protein Name Cystatin-C
Cystatin-3
Gamma-Trace
Neuroendocrine Basic Polypeptide
Post-Gamma-Globulin
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-6798695
Reactome: R-HSA-8957275
Reactome: R-HSA-977225
Cellular Localisation Secreted
Alternative ELISA Names Cystatin-C ELISA kit
Cystatin-3 ELISA kit
Gamma-Trace ELISA kit
Neuroendocrine Basic Polypeptide ELISA kit
Post-Gamma-Globulin ELISA kit
CST3 ELISA kit
Specificity This kit recognizes Human Cys-C in samples. No significant cross-reactivity or interference between Human Cys-C and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-105 98
EDTA plasma (n=8) 94-110 100
Cell culture media (n=8) 92-101 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.30 6.80 15.60 2.30 7.50 15.60
Standard deviation 0.09 0.12 0.23 0.10 0.16 0.24
CV (%) 4.55 4.22 5.28 6.13 5.73 4.29
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance