| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CSF2RB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CSF2RB |
| Gene ID: |
1439 |
| Uniprot ID: |
IL3RB_HUMAN |
| Specificity: |
This kit recognizes Human CSF2R Beta in samples. No significant cross-reactivity or interference between Human CSF2R Beta and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | May be phosphorylated by LYN. |
| Function | Cell surface receptor that plays a role in immune response and controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Acts by forming an heterodimeric receptor through interaction with different partners such as IL3RA, IL5RA or CSF2RA. In turn, participates in various signaling pathways including interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor/CSF2 pathways. In unstimulated conditions, interacts constitutively with JAK1 and ligand binding leads to JAK1 stimulation and subsequent activation of the JAK-STAT pathway. |
| Protein Name | Cytokine Receptor Common Subunit BetaCdw131Gm-Csf/Il-3/Il-5 Receptor Common Beta SubunitCd Antigen Cd131 |
| Database Links | Reactome: R-HSA-512988Reactome: R-HSA-5673001Reactome: R-HSA-5683826Reactome: R-HSA-5688849Reactome: R-HSA-5688890Reactome: R-HSA-912526 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative CLIA Names | Cytokine Receptor Common Subunit Beta CLIA kitCdw131 CLIA kitGm-Csf/Il-3/Il-5 Receptor Common Beta Subunit CLIA kitCd Antigen Cd131 CLIA kitCSF2RB CLIA kitIL3RB CLIA kitIL5RB CLIA kit |
| Specificity | This kit recognizes Human CSF2Rβ in samples. No significant cross-reactivity or interference between Human CSF2Rβ and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 98-115 | 106 |
| EDTA plasma(n=8) | 94-108 | 101 |
| Cell culture media(n=8) | 93-106 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 93.63 | 211.51 | 811.64 | 87.71 | 195.80 | 837.74 |
| Standard deviation | 11.33 | 20.37 | 63.31 | 11.18 | 18.15 | 58.39 |
| CV (%) | 12.10 | 9.63 | 7.80 | 12.75 | 9.27 | 6.97 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
