• Human CSF2 (Granulocyte-macrophage colony-stimulating factor) Sandwich ELISA (STJE0001974)

Human CSF2 (Granulocyte-macrophage colony-stimulating factor) Sandwich ELISA (STJE0001974)

SKU:
STJE0001974

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This CSF2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CSF2
Gene ID: 1437
Uniprot ID: CSF2_HUMAN
Specificity: This kit recognizes Human GM-CSF in samples. No significant cross-reactivity or interference between Human GM-CSF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Granulocyte-Macrophage Colony-Stimulating Factor
Gm-Csf
Colony-Stimulating Factor
Csf
Molgramostin
Sargramostim
Database Links Reactome: R-HSA-512988
Reactome: R-HSA-5673001
Reactome: R-HSA-6783783
Reactome: R-HSA-8939246
Reactome: R-HSA-912526
Cellular Localisation Secreted
Alternative ELISA Names Granulocyte-Macrophage Colony-Stimulating Factor ELISA kit
Gm-Csf ELISA kit
Colony-Stimulating Factor ELISA kit
Csf ELISA kit
Molgramostin ELISA kit
Sargramostim ELISA kit
CSF2 ELISA kit
GMCSF ELISA kit
Specificity This kit recognizes Human GM-CSF in samples. No significant cross-reactivity or interference between Human GM-CSF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 92
EDTA plasma (n=8) 88-102 94
Cell culture media (n=8) 88-101 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 25.18 75.65 183.61 25.92 75.14 168.57
Standard deviation 1.67 4.06 9.88 1.40 3.32 5.16
CV (%) 6.63 5.37 5.38 5.40 4.42 3.06
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance