| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.060ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This CRHR1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Tissue Specificity | Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe. |
| Post Translational Modifications | C-terminal Ser or Thr residues may be phosphorylated. Phosphorylation at Ser-330 by PKA prevents maximal coupling to Gq-protein, and thereby negatively regulates downstream signaling. |
| Function | G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli. |
| Protein Name | Corticotropin-Releasing Factor Receptor 1Crf-R-1Crf-R1Crfr-1Corticotropin-Releasing Hormone Receptor 1Crh-R-1Crh-R1 |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-418555 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinEndosomeAgonist-Binding Promotes Endocytosis |
| Alternative ELISA Names | Corticotropin-Releasing Factor Receptor 1 ELISA kitCrf-R-1 ELISA kitCrf-R1 ELISA kitCrfr-1 ELISA kitCorticotropin-Releasing Hormone Receptor 1 ELISA kitCrh-R-1 ELISA kitCrh-R1 ELISA kitCRHR1 ELISA kitCRFR ELISA kitCRFR1 ELISA kitCRHR ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
