• Human CRHBP (Corticotropin-releasing factor-binding protein) CLIA Kit (STJC0000685)

Human CRHBP (Corticotropin-releasing factor-binding protein) CLIA Kit (STJC0000685)

SKU:
STJC0000685

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human CRHBP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CRHBP
Gene ID: 1393
Uniprot ID: CRHBP_HUMAN
Specificity: This kit recognizes Human CRHBP in samples. No significant cross-reactivity or interference between Human CRHBP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy.
Protein Name Corticotropin-Releasing Factor-Binding Protein
Crf-Bp
Crf-Binding Protein
Corticotropin-Releasing Hormone-Binding Protein
Crh-Bp
Database Links Reactome: R-HSA-373080
Cellular Localisation Secreted
Alternative CLIA Names Corticotropin-Releasing Factor-Binding Protein CLIA kit
Crf-Bp CLIA kit
Crf-Binding Protein CLIA kit
Corticotropin-Releasing Hormone-Binding Protein CLIA kit
Crh-Bp CLIA kit
CRHBP CLIA kit
CRFBP CLIA kit
Specificity This kit recognizes Human CRHBP in samples. No significant cross-reactivity or interference between Human CRHBP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-104 97
EDTA plasma(n=8) 98-114 105
Cell culture media(n=8) 87-100 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 110.66 269.88 678.73 102.10 246.75 628.93
Standard deviation 11.59 22.51 61.56 12.12 18.83 64.40
CV (%) 10.47 8.34 9.07 11.87 7.63 10.24
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance