| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CORIN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CORIN |
| Gene ID: |
10699 |
| Uniprot ID: |
CORIN_HUMAN |
| Specificity: |
This kit recognizes Human CRN in samples. No significant cross-reactivity or interference between Human CRN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Highly expressed in heart. Expressed in heart myocytes. Also expressed in pregnant uterus. Detected in blood, in plasma as well as in serum (at protein level). |
| Post Translational Modifications | N-glycosylated.required for processing and activation. Activated through proteolytic processing by a trypsin-like protease.cleaved into a N-terminal propeptide and an activated corin protease fragment. Different soluble forms are produced by cleavage and autocatalytic cleavage: Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment is produced by cleavage by ADAM10, while 160 kDa and 100 kDa soluble fragments are produced by autocatalytic cleavage. Cleavage by ADAM10 to produce soluble 180 kDa soluble fragment takes place after the transmembrane region and before FZ 1. A disulfide bond links the activated corin protease fragment and the N-terminal propeptide. The disulfide bond also links the activated corin protease fragment with soluble fragments (100 kDa, 160 kDa and 180 kDa fragments). |
| Function | Serine-type endopeptidase involved in atrial natriuretic peptide (NPPA) and brain natriuretic peptide (NPPB) processing. Converts through proteolytic cleavage the non-functional propeptides NPPA and NPPB into their active hormones, ANP and BNP(1-32) respectively, thereby regulating blood pressure in the heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. Isoform 2: Has weaker endopeptidase activity compared to isoform 1. |
| Protein Name | Atrial Natriuretic Peptide-Converting EnzymeCorinHeart-Specific Serine Proteinase Atc2Pro-Anp-Converting EnzymeTransmembrane Protease Serine 10 Cleaved Into - Atrial Natriuretic Peptide-Converting Enzyme - N-Terminal Propeptide - Atrial Natriuretic Peptide-Converting Enzyme - Activated Protease Fragment - Atrial Natriuretic Peptide-Converting Enzyme - 180 Kda Soluble Fragment - Atrial Natriuretic Peptide-Converting Enzyme - 160 Kda Soluble Fragment - Atrial Natriuretic Peptide-Converting Enzyme - 100 Kda Soluble Fragment |
| Database Links | Reactome: R-HSA-5578768 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinMay Easily Detached From The Endothelial Cell MembraneIsoform 2: Cell MembraneLess Efficiently Targeted To The Cell Membrane Compared To Isoform 1Atrial Natriuretic Peptide-Converting Enzyme180 Kda Soluble Fragment: SecretedSoluble Form Produced Following Cleavage By Adam10160 Kda Soluble Fragment: SecretedSoluble Form Produced Following Autocatalytic Cleavage100 Kda Soluble Fragment: Secreted |
| Alternative CLIA Names | Atrial Natriuretic Peptide-Converting Enzyme CLIA kitCorin CLIA kitHeart-Specific Serine Proteinase Atc2 CLIA kitPro-Anp-Converting Enzyme CLIA kitTransmembrane Protease Serine 10 Cleaved Into - Atrial Natriuretic Peptide-Converting Enzyme - N-Terminal Propeptide - Atrial Natriuretic Peptide-Converting Enzyme - Activated Protease Fragment - Atrial Natriuretic Peptide-Converting Enzyme - 180 Kda Soluble Fragment - Atrial Natriuretic Peptide-Converting Enzyme - 160 Kda Soluble Fragment - Atrial Natriuretic Peptide-Converting Enzyme - 100 Kda Soluble Fragment CLIA kitCORIN CLIA kitCRN CLIA kitTMPRSS10 CLIA kit |
| Specificity | This kit recognizes Human CRN in samples. No significant cross-reactivity or interference between Human CRN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-100 | 91 |
| EDTA plasma(n=8) | 88-102 | 93 |
| Cell culture media(n=8) | 92-104 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 109.88 | 220.41 | 743.13 | 107.31 | 210.18 | 712.16 |
| Standard deviation | 9.42 | 20.32 | 48.30 | 11.78 | 20.14 | 66.94 |
| CV (%) | 8.57 | 9.22 | 6.50 | 10.98 | 9.58 | 9.40 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
