| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This COL4A1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
COL4A1 |
| Gene ID: |
1282 |
| Uniprot ID: |
CO4A1_HUMAN |
| Specificity: |
This kit recognizes Human COL4 Alpha 1 in samples. No significant cross-reactivity or interference between Human COL4 Alpha 1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated. The modified lysines can be O-glycosylated. Contains 4-hydroxyproline (Probable). Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Contains 3-hydroxyproline. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline. Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. The trimeric structure of the NC1 domains is stabilized by covalent bonds (sulfilimine cross-links) between Lys and Met residues. These cross-links are important for the mechanical stability of the basement membrane. Sulfilimine cross-link is catalyzed by PXDN. Proteolytic processing produces the C-terminal NC1 peptide, arresten. |
| Function | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. |
| Protein Name | Collagen Alpha-1(Iv Chain Cleaved Into - Arresten |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474244Reactome: R-HSA-1650814Reactome: R-HSA-186797Reactome: R-HSA-2022090Reactome: R-HSA-216083Reactome: R-HSA-2214320Reactome: R-HSA-2243919Reactome: R-HSA-3000157Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-3000480Reactome: R-HSA-419037Reactome: R-HSA-8948216 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement Membrane |
| Alternative ELISA Names | Collagen Alpha-1(Iv Chain Cleaved Into - Arresten ELISA kitCOL4A1 ELISA kit |
| Specificity | This kit recognizes Human COL4α1 in samples. No significant cross-reactivity or interference between Human COL4α1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-109 | 100 |
| EDTA plasma (n=8) | 93-108 | 101 |
| Cell culture media (n=8) | 87-103 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.07 | 2.74 | 7.57 | 1.16 | 2.93 | 7.95 |
| Standard deviation | 0.06 | 0.16 | 0.25 | 0.08 | 0.17 | 0.30 |
| CV (%) | 5.61 | 5.84 | 3.30 | 6.90 | 5.80 | 3.77 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
