| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CNTNAP2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CNTNAP2 |
| Gene ID: |
26047 |
| Uniprot ID: |
CNTP2_HUMAN |
| Specificity: |
This kit recognizes Human CNTNAP2 in samples. No significant cross-reactivity or interference between Human CNTNAP2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Predominantly expressed in nervous system. |
| Function | Required for gap junction formation (Probable). Required, with CNTNAP1, for radial and longitudinal organization of myelinated axons. Plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Demarcates the juxtaparanodal region of the axo-glial junction. |
| Protein Name | Contactin-Associated Protein-Like 2Cell Recognition Molecule Caspr2 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane ProteinCell ProjectionAxonCell JunctionParanodal Septate JunctionExpressed In The Juxtaparadonal Region |
| Alternative CLIA Names | Contactin-Associated Protein-Like 2 CLIA kitCell Recognition Molecule Caspr2 CLIA kitCNTNAP2 CLIA kitCASPR2 CLIA kitKIAA0868 CLIA kit |
| Specificity | This kit recognizes Human CNTNAP2 in samples. No significant cross-reactivity or interference between Human CNTNAP2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-102 | 93 |
| EDTA plasma(n=8) | 86-99 | 93 |
| Cell culture media(n=8) | 87-100 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 97.27 | 265.47 | 942.51 | 105.93 | 241.62 | 871.35 |
| Standard deviation | 10.05 | 23.92 | 78.04 | 10.08 | 28.00 | 71.54 |
| CV (%) | 10.33 | 9.01 | 8.28 | 9.52 | 11.59 | 8.21 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
