• Human CKB (Creatine kinase B-type) Sandwich ELISA (STJE0003710)

Human CKB (Creatine kinase B-type) Sandwich ELISA (STJE0003710)

SKU:
STJE0003710

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This CKB Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CKB
Gene ID: 1152
Uniprot ID: KCRB_HUMAN
Specificity: This kit recognizes Human CKB in samples. No significant cross-reactivity or interference between Human CKB and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Creatine Kinase B-Type
Brain Creatine Kinase
B-Ck
Creatine Kinase B Chain
Creatine Phosphokinase B-Type
Cpk-B
Database Links Reactome: R-HSA-71288
Reactome: R-HSA-9696264
Cellular Localisation Cytoplasm
Cytosol
Mitochondrion
Cell Membrane
Localizes To The Mitochondria Of Thermogenic Fat Cells Via The Internal Mts-Like Signal (Imts-L) Region
Alternative ELISA Names Creatine Kinase B-Type ELISA kit
Brain Creatine Kinase ELISA kit
B-Ck ELISA kit
Creatine Kinase B Chain ELISA kit
Creatine Phosphokinase B-Type ELISA kit
Cpk-B ELISA kit
CKB ELISA kit
CKBB ELISA kit
Specificity This kit recognizes Human CKB in samples. No significant cross-reactivity or interference between Human CKB and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-99 92
EDTA plasma (n=8) 86-99 93
Cell culture media (n=8) 93-108 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.02 3.18 7.58 1.03 3.11 8.09
Standard deviation 0.07 0.17 0.23 0.06 0.13 0.43
CV (%) 6.86 5.35 3.03 5.83 4.18 5.32
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance