| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This CFTR Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CFTR |
| Gene ID: |
1080 |
| Uniprot ID: |
CFTR_HUMAN |
| Specificity: |
This kit recognizes Human CFTR in samples. No significant cross-reactivity or interference between Human CFTR and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated. Phosphorylated.cAMP treatment promotes phosphorylation and activates the channel. Dephosphorylation decreases the ATPase activity (in vitro). Phosphorylation at PKA sites activates the channel. Phosphorylation at PKC sites enhances the response to phosphorylation by PKA. Phosphorylated by AMPK.this inhibits channel activity. Ubiquitinated, leading to its degradation in the lysosome. Deubiquitination by USP10 in early endosomes enhances its endocytic recycling to the cell membrane. Ubiquitinated by RNF185 during ER stress. Ubiquitinated by MARCHF2. |
| Function | Epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis. Mediates the transport of chloride ions across the cell membrane. Channel activity is coupled to ATP hydrolysis. The ion channel is also permeable to HCO(3)(-).selectivity depends on the extracellular chloride concentration. Exerts its function also by modulating the activity of other ion channels and transporters. Plays an important role in airway fluid homeostasis. Contributes to the regulation of the pH and the ion content of the airway surface fluid layer and thereby plays an important role in defense against pathogens. Modulates the activity of the epithelial sodium channel (ENaC) complex, in part by regulating the cell surface expression of the ENaC complex. Inhibits the activity of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G. Inhibits the activity of the ENaC channel containing subunits SCNN1D, SCNN1B and SCNN1G, but not of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the transporter SLC4A7. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation. |
| Protein Name | Cystic Fibrosis Transmembrane Conductance RegulatorCftrAtp-Binding Cassette Sub-Family C Member 7Channel Conductance-Controlling AtpaseCamp-Dependent Chloride Channel |
| Database Links | Reactome: R-HSA-382556Reactome: R-HSA-5627083Reactome: R-HSA-5678895Reactome: R-HSA-5689880Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-9013406Reactome: R-HSA-9613829Reactome: R-HSA-9615710Reactome: R-HSA-9646399 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinEarly Endosome MembraneCell MembraneRecycling Endosome MembraneEndoplasmic Reticulum MembraneNucleusThe Channel Is Internalized From The Cell Surface Into An Endosomal Recycling CompartmentFrom Where It Is Recycled To The Cell MembraneIn The Oviduct And BronchusDetected On The Apical Side Of Epithelial CellsBut Not Associated With CiliaIn Sertoli CellsA Processed Product Is Detected In The NucleusEr Stress Induces Gorasp2-Mediated Unconventional (Er/Golgi-Independent) Trafficking Of Core-Glycosylated Cftr To Cell Membrane |
| Alternative ELISA Names | Cystic Fibrosis Transmembrane Conductance Regulator ELISA kitCftr ELISA kitAtp-Binding Cassette Sub-Family C Member 7 ELISA kitChannel Conductance-Controlling Atpase ELISA kitCamp-Dependent Chloride Channel ELISA kitCFTR ELISA kitABCC7 ELISA kit |
| Specificity | This kit recognizes Human CFTR in samples. No significant cross-reactivity or interference between Human CFTR and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-95 | 90 |
| EDTA plasma (n=8) | 86-96 | 91 |
| Cell culture media (n=8) | 95-108 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.46 | 1.21 | 4.55 | 0.47 | 1.21 | 4.66 |
| Standard deviation | 0.03 | 0.07 | 0.19 | 0.03 | 0.05 | 0.18 |
| CV (%) | 6.52 | 5.79 | 4.18 | 6.38 | 4.13 | 3.86 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
