| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88ng/mL |
| Detection Limit: |
3.13~200ng/mL |
| Short Description: |
This CFI Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CFI |
| Gene ID: |
3426 |
| Uniprot ID: |
CFAI_HUMAN |
| Specificity: |
This kit recognizes Human CFI in samples. No significant cross-reactivity or interference between Human CFI and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins. Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces. The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization. |
| Protein Name | Complement Factor IC3b/C4b Inactivator Cleaved Into - Complement Factor I Heavy Chain - Complement Factor I Light Chain |
| Database Links | Reactome: R-HSA-977606 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative ELISA Names | Complement Factor I ELISA kitC3b/C4b Inactivator Cleaved Into - Complement Factor I Heavy Chain - Complement Factor I Light Chain ELISA kitCFI ELISA kitIF ELISA kit |
| Specificity | This kit recognizes Human CFI in samples. No significant cross-reactivity or interference between Human CFI and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 101-111 | 104 |
| EDTA plasma (n=8) | 91-103 | 98 |
| Cell culture media (n=8) | 90-102 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 8.43 | 20.17 | 94.33 | 8.12 | 21.37 | 82.88 |
| Standard deviation | 0.47 | 1.01 | 4.48 | 0.47 | 0.99 | 3.56 |
| CV (%) | 5.58 | 5.01 | 4.75 | 5.79 | 4.63 | 4.30 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
