Human CFH (Complement factor H) Sandwich ELISA (STJE0004069)

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STJE0004069

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 1.88ng/mL
Detection Limit: 3.13~200ng/mL
Short Description: This CFH Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CFH
Gene ID: 3075
Uniprot ID: CFAH_HUMAN
Specificity: This kit recognizes Human CFH in samples. No significant cross-reactivity or interference between Human CFH and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Sulfated on tyrosine residues.
Function Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed. (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination.
Protein Name Complement Factor H
H Factor 1
Database Links Reactome: R-HSA-977606
Cellular Localisation Secreted
(Microbial Infection) In The Mosquito Midgut
Localizes To P
Falciparum (Nf54 Strain) Macrogamete And Young Zygote Cell Membranes
Alternative ELISA Names Complement Factor H ELISA kit
H Factor 1 ELISA kit
CFH ELISA kit
HF ELISA kit
HF1 ELISA kit
HF2 ELISA kit
Specificity This kit recognizes Human CFH in samples. No significant cross-reactivity or interference between Human CFH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-94 91
EDTA plasma (n=8) 88-100 93
Cell culture media (n=8) 101-111 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 9.18 22.01 96.03 9.61 24.44 89.89
Standard deviation 0.53 1.03 4.38 0.57 1.22 4.54
CV (%) 5.77 4.68 4.56 5.93 4.99 5.05
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance