| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This CETP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CETP |
| Gene ID: |
1071 |
| Uniprot ID: |
CETP_HUMAN |
| Specificity: |
This kit recognizes Human CETP in samples. No significant cross-reactivity or interference between Human CETP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL. Regulates the reverse cholesterol transport, by which excess cholesterol is removed from peripheral tissues and returned to the liver for elimination. |
| Protein Name | Cholesteryl Ester Transfer ProteinLipid Transfer Protein I |
| Database Links | Reactome: R-HSA-8964041Reactome: R-HSA-8964058Reactome: R-HSA-9029569 |
| Cellular Localisation | SecretedSecreted In Plasma |
| Alternative ELISA Names | Cholesteryl Ester Transfer Protein ELISA kitLipid Transfer Protein I ELISA kitCETP ELISA kit |
| Specificity | This kit recognizes Human CETP in samples. No significant cross-reactivity or interference between Human CETP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 83-95 | 90 |
| EDTA plasma (n=8) | 84-99 | 90 |
| Cell culture media (n=8) | 86-99 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.01 | 2.37 | 9.20 | 1.03 | 2.44 | 10.07 |
| Standard deviation | 0.06 | 0.11 | 0.32 | 0.06 | 0.14 | 0.43 |
| CV (%) | 5.94 | 4.64 | 3.48 | 5.83 | 5.74 | 4.27 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
