| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This CDKN1B Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CDKN1B |
| Gene ID: |
1027 |
| Uniprot ID: |
CDN1B_HUMAN |
| Specificity: |
This kit recognizes Human P27 in samples. No significant cross-reactivity or interference between Human P27 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated.phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK1 and CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF. Ubiquitinated.in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated.by a TRIM21-containing SCF(SKP2)-like complex.leads to its degradation. Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation. |
| Function | Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. |
| Protein Name | Cyclin-Dependent Kinase Inhibitor 1bCyclin-Dependent Kinase Inhibitor P27P27kip1 |
| Database Links | Reactome: R-HSA-187577Reactome: R-HSA-198323Reactome: R-HSA-2559582Reactome: R-HSA-2559586Reactome: R-HSA-5625900Reactome: R-HSA-5674400Reactome: R-HSA-6804116Reactome: R-HSA-69202Reactome: R-HSA-69231Reactome: R-HSA-69563Reactome: R-HSA-69656Reactome: R-HSA-8849470Reactome: R-HSA-9607240Reactome: R-HSA-9617828Reactome: R-HSA-9634638Reactome: R-HSA-9661069 |
| Cellular Localisation | NucleusCytoplasmEndosomeNuclear And Cytoplasmic In Quiescent CellsAkt- Or Rsk-Mediated Phosphorylation On Thr-198Binds 14-3-3Translocates To The Cytoplasm And Promotes Cell Cycle ProgressionMitogen-Activated Uhmk1 Phosphorylation On Ser-10 Also Results In Translocation To The Cytoplasm And Cell Cycle ProgressionPhosphorylation On Ser-10 Facilitates Nuclear ExportTranslocates To The Nucleus On Phosphorylation Of Tyr-88 And Tyr-89Colocalizes At The Endosome With Snx6This Leads To Lysosomal Degradation |
| Alternative ELISA Names | Cyclin-Dependent Kinase Inhibitor 1b ELISA kitCyclin-Dependent Kinase Inhibitor P27 ELISA kitP27kip1 ELISA kitCDKN1B ELISA kitKIP1 ELISA kitp27 ELISA kit |
| Specificity | This kit recognizes Human P27 in samples. No significant cross-reactivity or interference between Human P27 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-100 | 93 |
| EDTA plasma (n=8) | 86-101 | 92 |
| Cell culture media (n=8) | 91-105 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 50.70 | 116.19 | 482.88 | 49.72 | 110.38 | 466.67 |
| Standard deviation | 3.49 | 5.29 | 18.54 | 2.64 | 5.20 | 24.64 |
| CV (%) | 6.88 | 4.55 | 3.84 | 5.31 | 4.71 | 5.28 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
