| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CD99 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CD99 |
| Gene ID: |
4267 |
| Uniprot ID: |
CD99_HUMAN |
| Specificity: |
This kit recognizes Human CD99 in samples. No significant cross-reactivity or interference between Human CD99 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Extensively O-glycosylated. |
| Function | Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. |
| Protein Name | Cd99 Antigen12e7E2 AntigenProtein Mic2T-Cell Surface Glycoprotein E2Cd Antigen Cd99 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-202733 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative CLIA Names | Cd99 Antigen CLIA kit12e7 CLIA kitE2 Antigen CLIA kitProtein Mic2 CLIA kitT-Cell Surface Glycoprotein E2 CLIA kitCd Antigen Cd99 CLIA kitCD99 CLIA kitMIC2 CLIA kitMIC2X CLIA kitMIC2Y CLIA kit |
| Specificity | This kit recognizes Human CD99 in samples. No significant cross-reactivity or interference between Human CD99 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-103 | 96 |
| EDTA plasma(n=8) | 93-105 | 99 |
| Cell culture media(n=8) | 89-100 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 106.99 | 180.43 | 878.54 | 100.27 | 182.93 | 954.30 |
| Standard deviation | 13.08 | 19.31 | 70.55 | 11.69 | 21.92 | 107.93 |
| CV (%) | 12.23 | 10.70 | 8.03 | 11.66 | 11.98 | 11.31 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
