| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.58 pg/mL |
| Detection Limit: |
7.81-500 pg/mL |
| Short Description: |
This CD63 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CD63 |
| Gene ID: |
967 |
| Uniprot ID: |
CD63_HUMAN |
| Specificity: |
This kit recognizes Human CD63 in samples. No significant cross-reactivity or interference between Human CD63 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. |
| Post Translational Modifications | Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). |
| Function | Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. |
| Protein Name | Cd63 AntigenGranulophysinLysosomal-Associated Membrane Protein 3Lamp-3Lysosome Integral Membrane Protein 1Limp1Melanoma-Associated Antigen Me491Oma81hOcular Melanoma-Associated AntigenTetraspanin-30Tspan-30Cd Antigen Cd63 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinLysosome MembraneLate Endosome MembraneEndosomeMultivesicular BodyMelanosomeSecretedExtracellular ExosomeCell SurfaceAlso Found In Weibel-Palade Bodies Of Endothelial CellsLocated In Platelet Dense GranulesDetected In A Subset Of Pre-MelanosomesDetected On Intralumenal Vesicles (Ilvs) Within Multivesicular Bodies |
| Alternative ELISA Names | Cd63 Antigen ELISA kitGranulophysin ELISA kitLysosomal-Associated Membrane Protein 3 ELISA kitLamp-3 ELISA kitLysosome Integral Membrane Protein 1 ELISA kitLimp1 ELISA kitMelanoma-Associated Antigen Me491 ELISA kitOma81h ELISA kitOcular Melanoma-Associated Antigen ELISA kitTetraspanin-30 ELISA kitTspan-30 ELISA kitCd Antigen Cd63 ELISA kitCD63 ELISA kitMLA1 ELISA kitTSPAN30 ELISA kit |
| Specificity | This kit recognizes Human CD63 in samples. No significant cross-reactivity or interference between Human CD63 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-105 | 99 |
| EDTA plasma (n=8) | 93-107 | 99 |
| Cell culture media (n=8) | 86-99 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 24.66 | 73.52 | 242.56 | 25.20 | 71.68 | 238.97 |
| Standard deviation | 1.68 | 3.37 | 12.98 | 1.34 | 3.48 | 8.15 |
| CV (%) | 6.80 | 4.59 | 5.35 | 5.32 | 4.86 | 3.41 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
