| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human CD59 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CD59 |
| Gene ID: |
966 |
| Uniprot ID: |
CD59_HUMAN |
| Specificity: |
This kit recognizes Human CD59 in samples. No significant cross-reactivity or interference between Human CD59 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine. Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants. Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. |
| Function | Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. |
| Protein Name | Cd59 Glycoprotein1f5 Antigen20 Kda Homologous Restriction FactorHrf-20Hrf20Mac-Inhibitory ProteinMac-IpMem43 AntigenMembrane Attack Complex Inhibition FactorMacifMembrane Inhibitor Of Reactive LysisMirlProtectinCd Antigen Cd59 |
| Database Links | Reactome: R-HSA-204005Reactome: R-HSA-5694530Reactome: R-HSA-6798695Reactome: R-HSA-6807878Reactome: R-HSA-977606 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorSecretedSoluble Form Found In A Number Of Tissues |
| Alternative CLIA Names | Cd59 Glycoprotein CLIA kit1f5 Antigen CLIA kit20 Kda Homologous Restriction Factor CLIA kitHrf-20 CLIA kitHrf20 CLIA kitMac-Inhibitory Protein CLIA kitMac-Ip CLIA kitMem43 Antigen CLIA kitMembrane Attack Complex Inhibition Factor CLIA kitMacif CLIA kitMembrane Inhibitor Of Reactive Lysis CLIA kitMirl CLIA kitProtectin CLIA kitCd Antigen Cd59 CLIA kitCD59 CLIA kitMIC11 CLIA kitMIN1 CLIA kitMIN2 CLIA kitMIN3 CLIA kitMSK21 CLIA kit |
| Specificity | This kit recognizes Human CD59 in samples. No significant cross-reactivity or interference between Human CD59 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-117 | 108 |
| EDTA plasma(n=8) | 96-111 | 104 |
| Cell culture media(n=8) | 100-117 | 107 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 54.60 | 120.88 | 475.96 | 54.10 | 109.30 | 441.40 |
| Standard deviation | 5.40 | 13.20 | 50.02 | 5.46 | 10.83 | 48.29 |
| CV (%) | 9.89 | 10.92 | 10.51 | 10.09 | 9.91 | 10.94 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
