| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This human CD38 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CD38 |
| Gene ID: |
952 |
| Uniprot ID: |
CD38_HUMAN |
| Specificity: |
This kit recognizes Human cADPRH/CD38 in samples. No significant cross-reactivity or interference between Human cADPRH/CD38 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. |
| Function | Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion. Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+). Has cADPR hydrolase activity. |
| Protein Name | Adp-Ribosyl Cyclase/Cyclic Adp-Ribose Hydrolase 12'-Phospho-Adp-Ribosyl Cyclase2'-Phospho-Adp-Ribosyl Cyclase/2'-Phospho-Cyclic-Adp-Ribose Transferase2'-Phospho-Cyclic-Adp-Ribose TransferaseAdp-Ribosyl Cyclase 1Adprc 1Cyclic Adp-Ribose Hydrolase 1Cadpr Hydrolase 1T10Cd Antigen Cd38 |
| Database Links | Reactome: R-HSA-196807 |
| Cellular Localisation | Cell SurfaceMembraneSingle-Pass Type Ii Membrane Protein |
| Alternative CLIA Names | Adp-Ribosyl Cyclase/Cyclic Adp-Ribose Hydrolase 1 CLIA kit2'-Phospho-Adp-Ribosyl Cyclase CLIA kit2'-Phospho-Adp-Ribosyl Cyclase/2'-Phospho-Cyclic-Adp-Ribose Transferase CLIA kit2'-Phospho-Cyclic-Adp-Ribose Transferase CLIA kitAdp-Ribosyl Cyclase 1 CLIA kitAdprc 1 CLIA kitCyclic Adp-Ribose Hydrolase 1 CLIA kitCadpr Hydrolase 1 CLIA kitT10 CLIA kitCd Antigen Cd38 CLIA kitCD38 CLIA kit |
| Specificity | This kit recognizes Human cADPRH/CD38 in samples. No significant cross-reactivity or interference between Human cADPRH/CD38 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-112 | 103 |
| EDTA plasma(n=8) | 97-113 | 104 |
| Cell culture media(n=8) | 99-112 | 105 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.08 | 2.73 | 8.03 | 1.07 | 2.76 | 8.15 |
| Standard deviation | 0.12 | 0.27 | 0.81 | 0.10 | 0.31 | 0.57 |
| CV (%) | 11.11 | 9.89 | 10.09 | 9.35 | 11.23 | 6.99 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
