| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This CD274 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CD274 |
| Gene ID: |
29126 |
| Uniprot ID: |
PD1L1_HUMAN |
| Specificity: |
This kit recognizes Human PD-L1 in samples. No significant cross-reactivity or interference between Human PD-L1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Ubiquitinated.STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation. Ubiquitinated by MARCHF8.leading to degradation. |
| Function | Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. |
| Protein Name | Programmed Cell Death 1 Ligand 1Pd-L1Pdcd1 Ligand 1Programmed Death Ligand 1Hpd-L1B7 Homolog 1B7-H1Cd Antigen Cd274 |
| Database Links | Reactome: R-HSA-389948Reactome: R-HSA-9701898 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEarly Endosome MembraneRecycling Endosome MembraneNucleusAssociates With Cmtm6 At Recycling EndosomesWhere It Is Protected From Being Targeted For Lysosomal DegradationTranslocates To The Nucleus In Response To Hypoxia Via Its Interaction With Phosphorylated Stat3Isoform 1: Cell MembraneIsoform 2: Endomembrane SystemIsoform 4: Secreted |
| Alternative ELISA Names | Programmed Cell Death 1 Ligand 1 ELISA kitPd-L1 ELISA kitPdcd1 Ligand 1 ELISA kitProgrammed Death Ligand 1 ELISA kitHpd-L1 ELISA kitB7 Homolog 1 ELISA kitB7-H1 ELISA kitCd Antigen Cd274 ELISA kitCD274 ELISA kitB7H1 ELISA kitPDCD1L1 ELISA kitPDCD1LG1 ELISA kitPDL1 ELISA kit |
| Specificity | This kit recognizes Human PD-L1 in samples. No significant cross-reactivity or interference between Human PD-L1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-111 | 102 |
| EDTA plasma (n=8) | 92-104 | 98 |
| Cell culture media (n=8) | 90-102 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.54 | 1.35 | 4.76 | 0.49 | 1.29 | 5.23 |
| Standard deviation | 0.03 | 0.08 | 0.17 | 0.03 | 0.05 | 0.18 |
| CV (%) | 5.56 | 5.93 | 3.57 | 6.12 | 3.88 | 3.44 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
