| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human CCN2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CCN2 |
| Gene ID: |
1490 |
| Uniprot ID: |
CCN2_HUMAN |
| Specificity: |
This kit recognizes Human CTGF in samples. No significant cross-reactivity or interference between Human CTGF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested. |
| Function | Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. |
| Protein Name | Ccn Family Member 2Cellular Communication Network Factor 2Connective Tissue Growth FactorHypertrophic Chondrocyte-Specific Protein 24Insulin-Like Growth Factor-Binding Protein 8Ibp-8Igf-Binding Protein 8Igfbp-8 |
| Database Links | Reactome: R-HSA-2032785Reactome: R-HSA-8951671 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative CLIA Names | Ccn Family Member 2 CLIA kitCellular Communication Network Factor 2 CLIA kitConnective Tissue Growth Factor CLIA kitHypertrophic Chondrocyte-Specific Protein 24 CLIA kitInsulin-Like Growth Factor-Binding Protein 8 CLIA kitIbp-8 CLIA kitIgf-Binding Protein 8 CLIA kitIgfbp-8 CLIA kitCCN2 CLIA kitCTGF CLIA kitHCS24 CLIA kitIGFBP8 CLIA kit |
| Specificity | This kit recognizes Human CTGF in samples. No significant cross-reactivity or interference between Human CTGF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-109 | 102 |
| EDTA plasma(n=8) | 91-105 | 97 |
| Cell culture media(n=8) | 98-110 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 51.99 | 107.29 | 487.85 | 52.58 | 96.98 | 520.00 |
| Standard deviation | 4.52 | 8.13 | 44.30 | 5.99 | 8.84 | 44.67 |
| CV (%) | 8.69 | 7.58 | 9.08 | 11.39 | 9.12 | 8.59 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
