| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This human CCL7 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CCL7 |
| Gene ID: |
6354 |
| Uniprot ID: |
CCL7_HUMAN |
| Specificity: |
This kit recognizes Human MCP-3 in samples. No significant cross-reactivity or interference between Human MCP-3 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | O-glycosylated. |
| Function | Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. |
| Protein Name | C-C Motif Chemokine 7Monocyte Chemoattractant Protein 3Monocyte Chemotactic Protein 3Mcp-3Nc28Small-Inducible Cytokine A7 |
| Database Links | Reactome: R-HSA-380108 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | C-C Motif Chemokine 7 CLIA kitMonocyte Chemoattractant Protein 3 CLIA kitMonocyte Chemotactic Protein 3 CLIA kitMcp-3 CLIA kitNc28 CLIA kitSmall-Inducible Cytokine A7 CLIA kitCCL7 CLIA kitMCP3 CLIA kitSCYA6 CLIA kitSCYA7 CLIA kit |
| Specificity | This kit recognizes Human MCP-3 in samples. No significant cross-reactivity or interference between Human MCP-3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-99 | 92 |
| EDTA plasma(n=8) | 85-97 | 91 |
| Cell culture media(n=8) | 93-106 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 25.30 | 53.26 | 234.51 | 26.68 | 54.06 | 254.40 |
| Standard deviation | 3.14 | 5.48 | 16.51 | 2.24 | 4.00 | 17.43 |
| CV (%) | 12.41 | 10.29 | 7.04 | 8.40 | 7.40 | 6.85 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
