| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This CCL3L1; CCL3L3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CCL3L1.CCL3L3 |
| Gene ID: |
414062/6349 |
| Uniprot ID: |
CL3L1_HUMAN |
| Specificity: |
This kit recognizes Human CCL3L1 in samples. No significant cross-reactivity or interference between Human CCL3L1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Chemotactic for lymphocytes and monocytes. Is a ligand for CCR1, CCR3 and CCR5. Is an inhibitor of HIV-1-infection. The processed form LD78-beta(3-70) shows a 20-fold to 30-fold higher chemotactic activity and is a very potent inhibitor of HIV-1-infection. LD78-beta(3-70) is also a ligand for CCR1, CCR3 and CCR5. |
| Protein Name | C-C Motif Chemokine 3-Like 1G0/G1 Switch Regulatory Protein 19-2Ld78-Beta(1-70Pat 464.2Small-Inducible Cytokine A3-Like 1Tonsillar Lymphocyte Ld78 Beta Protein Cleaved Into - Ld78-Beta(3-70 - Ld78-Beta(5-70 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-6783783 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | C-C Motif Chemokine 3-Like 1 ELISA kitG0/G1 Switch Regulatory Protein 19-2 ELISA kitLd78-Beta(1-70 ELISA kitPat 464.2 ELISA kitSmall-Inducible Cytokine A3-Like 1 ELISA kitTonsillar Lymphocyte Ld78 Beta Protein Cleaved Into - Ld78-Beta(3-70 - Ld78-Beta(5-70 ELISA kitCCL3L1 ELISA kitD17S1718 ELISA kitG0S19-2 ELISA kitSCYA3L1.CCL3L3 ELISA kit |
| Specificity | This kit recognizes Human CCL3L1 in samples. No significant cross-reactivity or interference between Human CCL3L1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-105 | 98 |
| EDTA plasma (n=8) | 87-98 | 93 |
| Cell culture media (n=8) | 85-99 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 230.19 | 696.41 | 2213.50 | 215.95 | 724.84 | 2270.93 |
| Standard deviation | 12.06 | 39.07 | 120.19 | 11.81 | 31.31 | 77.89 |
| CV (%) | 5.24 | 5.61 | 5.43 | 5.47 | 4.32 | 3.43 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
