| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human CCL26 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CCL26 |
| Gene ID: |
10344 |
| Uniprot ID: |
CCL26_HUMAN |
| Specificity: |
This kit recognizes Human MIP4 Alpha in samples. No significant cross-reactivity or interference between Human MIP4 Alpha and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Ubiquitously expressed at low levels in various tissues including heart and ovary. |
| Function | Chemoattractant for eosinophils and basophils. Acts as a ligand for C-C chemokine receptor CCR3 which triggers Ca(2+) mobilization in eosinophils. Also acts as a ligand for CX3C chemokine receptor CX3CR1, inducing cell chemotaxis. |
| Protein Name | C-C Motif Chemokine 26Cc Chemokine ImacEotaxin-3Macrophage Inflammatory Protein 4-AlphaMip-4-AlphaSmall-Inducible Cytokine A26Thymic Stroma Chemokine-1Tsc-1 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | C-C Motif Chemokine 26 CLIA kitCc Chemokine Imac CLIA kitEotaxin-3 CLIA kitMacrophage Inflammatory Protein 4-Alpha CLIA kitMip-4-Alpha CLIA kitSmall-Inducible Cytokine A26 CLIA kitThymic Stroma Chemokine-1 CLIA kitTsc-1 CLIA kitCCL26 CLIA kitSCYA26 CLIA kitUNQ216 CLIA kitPRO242 CLIA kit |
| Specificity | This kit recognizes Human MIP4α in samples. No significant cross-reactivity or interference between Human MIP4α and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-108 | 103 |
| EDTA plasma(n=8) | 99-117 | 106 |
| Cell culture media(n=8) | 91-102 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 47.88 | 91.75 | 382.74 | 43.80 | 96.24 | 368.42 |
| Standard deviation | 4.95 | 7.32 | 30.58 | 5.46 | 8.88 | 42.26 |
| CV (%) | 10.34 | 7.98 | 7.99 | 12.47 | 9.23 | 11.47 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
