• Human CASP4 (Caspase-4) CLIA Kit (STJC0000357)

Human CASP4 (Caspase-4) CLIA Kit (STJC0000357)

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STJC0000357

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human CASP4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CASP4
Gene ID: 837
Uniprot ID: CASP4_HUMAN
Specificity: This kit recognizes Human CASP4 in samples. No significant cross-reactivity or interference between Human CASP4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). Not detected in brain.
Post Translational Modifications In response to activation signals, including endoplasmic reticulum stress or treatment with amyloid-beta A4 protein fragments (such as amyloid-beta protein 40), undergoes autoproteolytic cleavage. (Microbial infection) ADP-riboxanation by S.flexneri OspC3 blocks CASP4 autoprocessing, preventing CASP4 activation and ability to recognize and cleave GSDMD, thereby thwarting the inflammasome/pyroptosis-mediated defense.
Function Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis. Also indirectly activates the NLRP3 and NLRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS, GSDMD and IL18. Effector of the non-canonical inflammasome independently of NLRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine IL1B and IL18. In the non-canonical inflammasome, CASP4 is activated by direct binding to LPS without the need of an upstream sensor. LPS-binding promotes CASP4 activation and CASP4-mediated cleavage of GSDMD, followed by pyroptosis of infected cells and their extrusion into the gut lumen. Also indirectly promotes secretion of mature cytokines (IL1A, IL18 and HMGB1) downstream of GSDMD-mediated pyroptosis via activation of the NLRP3 and NLRP6 inflammasomes. Involved in NLRP3-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation or cholera enterotoxin. Involved in NLRP6 inflammasome-dependent activation in response to lipoteichoic acid (LTA), a cell-wall component of Gram-positive bacteria, which leads to CASP1 activation and IL1B and IL18 secretion. Involved in LPS-induced IL6 secretion.this activity may not require caspase enzymatic activity. The non-canonical inflammasome is required for innate immunity to cytosolic, but not vacuolar, bacteria. Plays a crucial role in the restriction of S.typhimurium replication in colonic epithelial cells during infection. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. May also act as an activator of adaptive immunity in dendritic cells, following activation by oxidized phospholipid 1-palmitoyl-2-arachidonoyl- sn-glycero-3-phosphorylcholine, an oxidized phospholipid (oxPAPC). Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found in Alzheimer's patient brains. Cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Catalyzes cleavage and maturation of IL18. In contrast, it does not directly process IL1B. During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation. (Microbial infection) In response to the Td92 surface protein of the periodontal pathogen T.denticola, activated by cathepsin CTSG which leads to production and secretion of IL1A and pyroptosis of gingival fibroblasts.
Protein Name Caspase-4
Casp-4
Ice And Ced-3 Homolog 2
Ich-2
Ice(Rel-Ii
Mih1
Protease Tx Cleaved Into - Caspase-4 Subunit P10 - Caspase-4 Subunit P20
Database Links Reactome: R-HSA-168638
Reactome: R-HSA-5620971
Cellular Localisation Cytoplasm
Cytosol
Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Mitochondrion
Inflammasome
Secreted
Predominantly Localizes To The Endoplasmic Reticulum (Er)
Association With The Er Membrane Requires Tmem214
Released In The Extracellular Milieu By Keratinocytes Following Uvb Irradiation
Alternative CLIA Names Caspase-4 CLIA kit
Casp-4 CLIA kit
Ice And Ced-3 Homolog 2 CLIA kit
Ich-2 CLIA kit
Ice(Rel-Ii CLIA kit
Mih1 CLIA kit
Protease Tx Cleaved Into - Caspase-4 Subunit P10 - Caspase-4 Subunit P20 CLIA kit
CASP4 CLIA kit
ICH2 CLIA kit
Specificity This kit recognizes Human CASP4 in samples. No significant cross-reactivity or interference between Human CASP4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-96 90
EDTA plasma(n=8) 94-107 100
Cell culture media(n=8) 101-116 107

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 103.90 217.33 887.52 102.82 203.34 879.17
Standard deviation 9.13 20.97 81.83 11.70 15.47 90.20
CV (%) 8.79 9.65 9.22 11.38 7.61 10.26
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance