| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.064ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This CAMK2a Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | CAMK2A |
| Gene ID: | 815 |
| Uniprot ID: | KCC2A_HUMAN |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state. Palmitoylated. Probably palmitoylated by ZDHHC3 and ZDHHC7. |
| Function | Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission. Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity. Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway. In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway. Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity. |
| Protein Name | Calcium/Calmodulin-Dependent Protein Kinase Type Ii Subunit AlphaCam Kinase Ii Subunit AlphaCamk-Ii Subunit Alpha |
| Database Links | Reactome: R-HSA-111932Reactome: R-HSA-3371571Reactome: R-HSA-399719Reactome: R-HSA-4086398Reactome: R-HSA-438066Reactome: R-HSA-442982Reactome: R-HSA-5576892Reactome: R-HSA-5578775Reactome: R-HSA-5673000Reactome: R-HSA-5673001Reactome: R-HSA-6802946Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-877300Reactome: R-HSA-9022692Reactome: R-HSA-936837Reactome: R-HSA-9609736Reactome: R-HSA-9617324Reactome: R-HSA-9620244Reactome: R-HSA-9649948Reactome: R-HSA-9656223 |
| Cellular Localisation | SynapsePostsynaptic DensityCell ProjectionDendritic SpineDendritePostsynaptic Lipid Rafts |
| Alternative ELISA Names | Calcium/Calmodulin-Dependent Protein Kinase Type Ii Subunit Alpha ELISA kitCam Kinase Ii Subunit Alpha ELISA kitCamk-Ii Subunit Alpha ELISA kitCAMK2A ELISA kitCAMKA ELISA kitKIAA0968 ELISA kit |
| output |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
