| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CALU kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CALU |
| Gene ID: |
813 |
| Uniprot ID: |
CALU_HUMAN |
| Specificity: |
This kit recognizes Human CALU in samples. No significant cross-reactivity or interference between Human CALU and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Ubiquitously expressed. Expressed at high levels in heart, placenta and skeletal muscle, at lower levels in lung, kidney and pancreas and at very low levels in brain and liver. |
| Function | Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity. |
| Protein Name | CalumeninCrocalbinIef Ssp 9302 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Endoplasmic Reticulum MembraneGolgi ApparatusSecretedMelanosomeSarcoplasmic Reticulum LumenIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative CLIA Names | Calumenin CLIA kitCrocalbin CLIA kitIef Ssp 9302 CLIA kitCALU CLIA kit |
| Specificity | This kit recognizes Human CALU in samples. No significant cross-reactivity or interference between Human CALU and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-104 | 96 |
| EDTA plasma(n=8) | 92-104 | 99 |
| Cell culture media(n=8) | 98-113 | 105 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 103.30 | 211.14 | 703.75 | 100.04 | 214.83 | 751.22 |
| Standard deviation | 8.58 | 20.73 | 62.21 | 8.62 | 24.30 | 48.30 |
| CV (%) | 8.31 | 9.82 | 8.84 | 8.62 | 11.31 | 6.43 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
