| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This CALR Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CALR |
| Gene ID: |
811 |
| Uniprot ID: |
CALR_HUMAN |
| Specificity: |
This kit recognizes Human CRT in samples. No significant cross-reactivity or interference between Human CRT and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Protein Name | CalreticulinCrp55CalregulinEndoplasmic Reticulum Resident Protein 60Erp60HacbpGrp60 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-168316Reactome: R-HSA-3000480Reactome: R-HSA-3000484Reactome: R-HSA-381183Reactome: R-HSA-901042Reactome: R-HSA-983170 |
| Cellular Localisation | Endoplasmic Reticulum LumenCytoplasmCytosolSecretedExtracellular SpaceExtracellular MatrixCell SurfaceSarcoplasmic Reticulum LumenCytoplasmic VesicleSecretory VesicleCortical GranuleCytolytic GranuleAlso Found In Cell Surface (T Cells)Cytosol And Extracellular MatrixDuring Oocyte Maturation And After Parthenogenetic Activation Accumulates In Cortical GranulesIn Pronuclear And Early Cleaved Embryos Localizes Weakly To Cytoplasm Around Nucleus And More Strongly In The Region Near The CortexIn Cortical Granules Of Non-Activated OocytesIs Exocytosed During The Cortical Reaction In Response To Oocyte Activation |
| Alternative ELISA Names | Calreticulin ELISA kitCrp55 ELISA kitCalregulin ELISA kitEndoplasmic Reticulum Resident Protein 60 ELISA kitErp60 ELISA kitHacbp ELISA kitGrp60 ELISA kitCALR ELISA kitCRTC ELISA kit |
| Specificity | This kit recognizes Human CRT in samples. No significant cross-reactivity or interference between Human CRT and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-105 | 99 |
| EDTA plasma (n=8) | 86-98 | 91 |
| Cell culture media (n=8) | 90-105 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.53 | 1.36 | 4.94 | 0.52 | 1.31 | 4.68 |
| Standard deviation | 0.03 | 0.06 | 0.26 | 0.03 | 0.06 | 0.16 |
| CV (%) | 5.66 | 4.41 | 5.26 | 5.77 | 4.58 | 3.42 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
