| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human C1QBP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
C1QBP |
| Gene ID: |
708 |
| Uniprot ID: |
C1QBP_HUMAN |
| Specificity: |
This kit recognizes Human HABP1/C1QBP in samples. No significant cross-reactivity or interference between Human HABP1/C1QBP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed on cell surface of peripheral blood cells (at protein level).Surface expression is reported for macrophages and monocyte-derived dendritic cells. |
| Function | Is believed to be a multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, protein synthesis in mitochondria, regulation of apoptosis, transcriptional regulation and pre-mRNA splicing. At the cell surface is thought to act as an endothelial receptor for plasma proteins of the complement and kallikrein-kinin cascades. Putative receptor for C1q.specifically binds to the globular 'heads' of C1q thus inhibiting C1.may perform the receptor function through a complex with C1qR/CD93. In complex with cytokeratin-1/KRT1 is a high affinity receptor for kininogen-1/HMWK. Can also bind other plasma proteins, such as coagulation factor XII leading to its autoactivation. May function to bind initially fluid kininogen-1 to the cell membrane. The secreted form may enhance both extrinsic and intrinsic coagulation pathways. It is postulated that the cell surface form requires docking with transmembrane proteins for downstream signaling which might be specific for a cell-type or response. By acting as C1q receptor is involved in chemotaxis of immature dendritic cells and neutrophils and is proposed to signal through CD209/DC-SIGN on immature dendritic cells, through integrin alpha-4/beta-1 during trophoblast invasion of the decidua, and through integrin beta-1 during endothelial cell adhesion and spreading. Signaling involved in inhibition of innate immune response is implicating the PI3K-AKT/PKB pathway. Required for protein synthesis in mitochondria. In mitochondrial translation may be involved in formation of functional 55S mitoribosomes.the function seems to involve its RNA-binding activity. May be involved in the nucleolar ribosome maturation process.the function may involve the exchange of FBL for RRP1 in the association with pre-ribosome particles. Involved in regulation of RNA splicing by inhibiting the RNA-binding capacity of SRSF1 and its phosphorylation. Is required for the nuclear translocation of splicing factor U2AF1L4. Involved in regulation of CDKN2A- and HRK-mediated apoptosis. Stabilizes mitochondrial CDKN2A isoform smARF. May be involved in regulation of FOXC1 transcriptional activity and NFY/CCAAT-binding factor complex-mediated transcription. May play a role in antibacterial defense as it can bind to cell surface hyaluronan and inhibit Streptococcus pneumoniae hyaluronate lyase. May be involved in modulation of the immune response.ligation by HCV core protein is resulting in suppression of interleukin-12 production in monocyte-derived dendritic cells. Involved in regulation of antiviral response by inhibiting RIGI- and IFIH1-mediated signaling pathways probably involving its association with MAVS after viral infection. (Microbial infection) Involved in HIV-1 replication, presumably by contributing to splicing of viral RNA. (Microbial infection) In infection processes acts as an attachment site for microbial proteins, including Listeria monocytogenes internalin B (InlB) and Staphylococcus aureus protein A. (Microbial infection) Involved in replication of Rubella virus. |
| Protein Name | Complement Component 1 Q Subcomponent-Binding Protein - MitochondrialAsf/Sf2-Associated Protein P32Glycoprotein Gc1qbpC1qbpHyaluronan-Binding Protein 1Mitochondrial Matrix Protein P32Gc1q-R ProteinP33Sf2ap32 |
| Database Links | Reactome: R-HSA-111471Reactome: R-HSA-140837Reactome: R-HSA-8980692Reactome: R-HSA-9013106Reactome: R-HSA-9645722 |
| Cellular Localisation | Mitochondrion MatrixNucleusNucleolusCell MembranePeripheral Membrane ProteinExtracellular SideSecretedCytoplasmSeems To Be Predominantly Localized To MitochondriaSecreted By Activated LymphocytesLocalizes To The Nucleolus When Coexpressed With PolgarfInteraction With Polgarf Is Likely To Result In Prevention Of C1qbp Maturation And Redirection From Mitochondria To Nucleoli |
| Alternative CLIA Names | Complement Component 1 Q Subcomponent-Binding Protein - Mitochondrial CLIA kitAsf/Sf2-Associated Protein P32 CLIA kitGlycoprotein Gc1qbp CLIA kitC1qbp CLIA kitHyaluronan-Binding Protein 1 CLIA kitMitochondrial Matrix Protein P32 CLIA kitGc1q-R Protein CLIA kitP33 CLIA kitSf2ap32 CLIA kitC1QBP CLIA kitGC1QBP CLIA kitHABP1 CLIA kitSF2P32 CLIA kit |
| Specificity | This kit recognizes Human HABP1/C1QBP in samples. No significant cross-reactivity or interference between Human HABP1/C1QBP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-99 | 94 |
| EDTA plasma(n=8) | 94-110 | 101 |
| Cell culture media(n=8) | 94-108 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 100.39 | 290.08 | 811.44 | 105.22 | 297.49 | 850.24 |
| Standard deviation | 8.95 | 27.47 | 53.72 | 12.25 | 23.77 | 67.51 |
| CV (%) | 8.92 | 9.47 | 6.62 | 11.64 | 7.99 | 7.94 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
