| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.059ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This Btk Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
BTK |
| Gene ID: |
695 |
| Uniprot ID: |
BTK_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Predominantly expressed in B-lymphocytes. |
| Post Translational Modifications | Following B-cell receptor (BCR) engagement, translocates to the plasma membrane where it gets phosphorylated at Tyr-551 by LYN and SYK. Phosphorylation at Tyr-551 is followed by autophosphorylation of Tyr-223 which may create a docking site for a SH2 containing protein. Phosphorylation at Ser-180 by PRKCB, leads in translocation of BTK back to the cytoplasmic fraction. Phosphorylation at Ser-21 and Ser-115 creates a binding site for PIN1 at these Ser-Pro motifs, and promotes it's recruitment. |
| Function | Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK also plays a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Acts as an activator of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis. |
| Protein Name | Tyrosine-Protein Kinase BtkAgammaglobulinemia Tyrosine KinaseAtkB-Cell Progenitor KinaseBpkBruton Tyrosine Kinase |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-166058Reactome: R-HSA-2029482Reactome: R-HSA-2424491Reactome: R-HSA-2871809Reactome: R-HSA-416476Reactome: R-HSA-416482Reactome: R-HSA-5602498Reactome: R-HSA-5603041Reactome: R-HSA-5663213Reactome: R-HSA-8964315Reactome: R-HSA-9664422Reactome: R-HSA-9679191Reactome: R-HSA-983695 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane ProteinNucleusMembrane RaftIn Steady StateBtk Is Predominantly CytosolicFollowing B-Cell Receptor (Bcr) Engagement By AntigenTranslocates To The Plasma Membrane Through Its Ph DomainPlasma Membrane Localization Is A Critical Step In The Activation Of BtkA Fraction Of Btk Also Shuttles Between The Nucleus And The CytoplasmAnd Nuclear Export Is Mediated By The Nuclear Export Receptor Crm1 |
| Alternative ELISA Names | Tyrosine-Protein Kinase Btk ELISA kitAgammaglobulinemia Tyrosine Kinase ELISA kitAtk ELISA kitB-Cell Progenitor Kinase ELISA kitBpk ELISA kitBruton Tyrosine Kinase ELISA kitBTK ELISA kitAGMX1 ELISA kitATK ELISA kitBPK ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
