Human BMP6 (Bone morphogenetic protein 6) Sandwich ELISA (STJE0004058)

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STJE0004058

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This BMP6 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: BMP6
Gene ID: 654
Uniprot ID: BMP6_HUMAN
Specificity: This kit recognizes Human BMP-6 in samples. No significant cross-reactivity or interference between Human BMP-6 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes including cartilage and bone formation. Also plays an important role in the regulation of HAMP/hepcidin expression and iron metabolism by acting as a ligand for hemojuvelin/HJV. Also acts to promote expression of HAMP, potentially via the interaction with its receptor BMPR1A/ALK3. Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2B. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target. Can also signal through non-canonical pathway such as TAZ-Hippo signaling cascade to modulate VEGF signaling by regulating VEGFR2 expression.
Protein Name Bone Morphogenetic Protein 6
Bmp-6
Vg-1-Related Protein
Vg-1-R
Vgr-1
Cellular Localisation Secreted
Alternative ELISA Names Bone Morphogenetic Protein 6 ELISA kit
Bmp-6 ELISA kit
Vg-1-Related Protein ELISA kit
Vg-1-R ELISA kit
Vgr-1 ELISA kit
BMP6 ELISA kit
VGR ELISA kit
Specificity This kit recognizes Human BMP-6 in samples. No significant cross-reactivity or interference between Human BMP-6 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-108 100
EDTA plasma (n=8) 95-111 103
Cell culture media (n=8) 92-101 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 40.09 122.13 458.34 41.53 105.65 456.97
Standard deviation 2.14 5.63 24.11 2.17 4.84 24.13
CV (%) 5.34 4.61 5.26 5.23 4.58 5.28
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance