| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human BID kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
BID |
| Gene ID: |
637 |
| Uniprot ID: |
BID_HUMAN |
| Specificity: |
This kit recognizes Human Bid in samples. No significant cross-reactivity or interference between Human Bid and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Isoform 2: Expressed in spleen, pancreas and placenta (at protein level). Isoform 3: Expressed in lung, pancreas and spleen (at protein level). Isoform 4: Expressed in lung and pancreas (at protein level). |
| Post Translational Modifications | BH3-interacting domain death agonist: TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products. Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis. BH3-interacting domain death agonist p15: Ubiquitinated by ITCH.ubiquitination results in proteasome-dependent degradation. |
| Function | Induces caspases and apoptosis. Counters the protective effect of BCL2. BH3-interacting domain death agonist p15: Induces caspase activation and apoptosis. Allows the release of cytochrome c. Isoform 1: Induces ICE-like proteases and apoptosis. Isoform 2: Induces ICE-like proteases and apoptosis. Isoform 3: Does not induce apoptosis. Isoform 4: Induces ICE-like proteases and apoptosis. |
| Protein Name | Bh3-Interacting Domain Death AgonistP22 BidBid Cleaved Into - Bh3-Interacting Domain Death Agonist P15P15 Bid - Bh3-Interacting Domain Death Agonist P13P13 Bid - Bh3-Interacting Domain Death Agonist P11P11 Bid |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-111452Reactome: R-HSA-111453Reactome: R-HSA-114294Reactome: R-HSA-6803204Reactome: R-HSA-75108 |
| Cellular Localisation | CytoplasmMitochondrion MembraneMitochondrion Outer MembraneWhen UncleavedIt Is Predominantly CytoplasmicBh3-Interacting Domain Death Agonist P15: Mitochondrion MembraneTranslocates To Mitochondria As An Integral Membrane ProteinBh3-Interacting Domain Death Agonist P13: Mitochondrion MembraneAssociated With The Mitochondrial MembraneIsoform 1: CytoplasmIsoform 3: CytoplasmIsoform 2: Mitochondrion MembraneA Significant Proportion Of Isoform 2 Localizes To MitochondriaIt May Be Cleaved Constitutively |
| Alternative CLIA Names | Bh3-Interacting Domain Death Agonist CLIA kitP22 Bid CLIA kitBid Cleaved Into - Bh3-Interacting Domain Death Agonist P15 CLIA kitP15 Bid - Bh3-Interacting Domain Death Agonist P13 CLIA kitP13 Bid - Bh3-Interacting Domain Death Agonist P11 CLIA kitP11 Bid CLIA kitBID CLIA kit |
| Specificity | This kit recognizes Human Bid in samples. No significant cross-reactivity or interference between Human Bid and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-102 | 95 |
| EDTA plasma(n=8) | 99-112 | 105 |
| Cell culture media(n=8) | 97-112 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 192.26 | 496.06 | 1924.59 | 196.70 | 537.74 | 1826.19 |
| Standard deviation | 17.28 | 36.96 | 171.67 | 24.82 | 57.11 | 151.39 |
| CV (%) | 8.99 | 7.45 | 8.92 | 12.62 | 10.62 | 8.29 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
