| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.024 ng/mL |
| Detection Limit: |
0078-5 ng/mL |
| Short Description: |
This Bax Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Conjugation: |
Biotin |
| Storage Instruction: |
Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month. |
| Assay Time: |
4 hours |
| Detection: |
Colorimetric |
| Gene Symbol: |
BAX |
| Gene ID: |
581 |
| Uniprot ID: |
BAX_HUMAN |
| Sample Type: |
serum, plasma and cell culture supernates |
| Tissue Specificity | Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. |
| Post Translational Modifications | Ubiquitinated on Lys-128 and Lys-190. 'Lys-63'-linked polyubiquitin chains on Lys-128 are removed by USP12. |
| Function | Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. |
| Protein Name | Apoptosis Regulator BaxBcl-2-Like Protein 4Bcl2-L-4 |
| Database Links | Reactome: R-HSA-111457Reactome: R-HSA-114294Reactome: R-HSA-5620971Reactome: R-HSA-6803204Reactome: R-HSA-6804114Reactome: R-HSA-8878166Reactome: R-HSA-9603505 |
| Cellular Localisation | Isoform Alpha: Mitochondrion Outer MembraneSingle-Pass Membrane ProteinCytoplasmNucleusColocalizes With 14-3-3 Proteins In The CytoplasmUnder Stress ConditionsUndergoes A Conformation Change That Causes Release From Jnk-Phosphorylated 14-3-3 Proteins And Translocation To The Mitochondrion MembraneUpon Sendai Virus InfectionRecruited To The Mitochondrion Through Interaction With Irf3Isoform Beta: CytoplasmIsoform Gamma: CytoplasmIsoform Delta: Cytoplasm |
| Alternative ELISA Names | Apoptosis Regulator Bax ELISA kitBcl-2-Like Protein 4 ELISA kitBcl2-L-4 ELISA kitBAX ELISA kitBCL2L4 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
