| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This human BAK1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
BAK1 |
| Gene ID: |
578 |
| Uniprot ID: |
BAK_HUMAN |
| Specificity: |
This kit recognizes Human BAK1 in samples. No significant cross-reactivity or interference between Human BAK1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. |
| Function | Plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases. |
| Protein Name | Bcl-2 Homologous Antagonist/KillerApoptosis Regulator BakBcl-2-Like Protein 7Bcl2-L-7 |
| Database Links | Reactome: R-HSA-111452Reactome: R-HSA-111457Reactome: R-HSA-5620971 |
| Cellular Localisation | Mitochondrion Outer MembraneSingle-Pass Membrane Protein |
| Alternative CLIA Names | Bcl-2 Homologous Antagonist/Killer CLIA kitApoptosis Regulator Bak CLIA kitBcl-2-Like Protein 7 CLIA kitBcl2-L-7 CLIA kitBAK1 CLIA kitBAK CLIA kitBCL2L7 CLIA kitCDN1 CLIA kit |
| Specificity | This kit recognizes Human BAK1 in samples. No significant cross-reactivity or interference between Human BAK1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 101-115 | 107 |
| EDTA plasma(n=8) | 85-96 | 90 |
| Cell culture media(n=8) | 92-108 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.47 | 0.84 | 4.13 | 0.50 | 0.83 | 3.95 |
| Standard deviation | 0.06 | 0.08 | 0.44 | 0.06 | 0.08 | 0.32 |
| CV (%) | 12.77 | 9.52 | 10.65 | 12.00 | 9.64 | 8.10 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
