| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.060ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This ASTL Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
ASTL |
| Gene ID: |
431705 |
| Uniprot ID: |
ASTL_HUMAN |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding. |
| Protein Name | Astacin-Like MetalloendopeptidaseOocyte AstacinOvastacinZp2-Proteinase |
| Database Links | |
| Cellular Localisation | CytoplasmCell MembraneCytoplasmic VesicleSecretory VesicleCortical GranuleProbably Exocytosed From Cortical Granules During Post-FertilizationDetected Throughout The Ooplasm Of Germinal Vesicle Stage Oocytes In Early Bilaminar Secondary Follicles At Postnatal (Pn) Day 3Detected In The Microvillar Domain Of The Oolemma In Arrested Ovulated Secondary Oocytes And In The First Polar Body Prior To FertilizationUpon FertilizationDetected In The Perivitelline Space (Pvs) And Occasionally On The Oolemma In 2-Cell Through Morulae StagesColocalizes With Spaca3 At The Microvillar Domain Of The Oolemma And In The Perivitelline Space (Pvs) |
| Alternative ELISA Names | Astacin-Like Metalloendopeptidase ELISA kitOocyte Astacin ELISA kitOvastacin ELISA kitZp2-Proteinase ELISA kitASTL ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
